First detection of influenza A virus genes from wild raccoons in Japan
First detection of influenza A virus genes from wild raccoons in Japan
复制标题
日本首次从野生浣熊身上检测到甲型流感病毒基因
DOI：
10.1007/s11262-018-1566-z
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发表时间：
2018
期刊：
影响因子：
1.6
通讯作者：
Imai Kunitoshi
中科院分区：
文献类型：
--
作者：
Yamaguchi Emi;Fujii Kei;Ogawa Haruko;Imai Kunitoshi
血清学调查显示，野生浣熊会接触甲型流感病毒（IAV）；然而，尚未发现这种 IAV 感染的遗传证据。在本研究中，我们首先在日本迁徙水禽越冬季节和流感流行季节以外捕获的野生浣熊中检测到IAV基因。通过传统的逆转录聚合酶链式反应测定，从三个哺乳期兄弟姐妹和一个青少年身上检测到病毒基质（M）和核蛋白（NP）基因，没有任何明显的临床症状，但无法从其中一个兄弟姐妹中检测到 NP 基因。从三个哺乳兄弟姐妹的直肠拭子中检测到的 M 基因片段序列彼此具有可比性，但与从不同地点捕获的幼年浣熊的鼻拭子中检测到的序列不同。从两个哺乳兄弟姐妹中检测到的 NP 基因片段序列也具有可比性。这些遗传证据表明，日本北部的浣熊种群中存在 IAV。需要对野生浣熊 IAV 感染进行进一步的遗传和病毒学研究，以更好地了解该领域的 IAV 生态。
Serological surveys have shown that wild raccoons are exposed to influenza A viruses (IAVs); however, no genetic evidence for this IAV infection has been found. In the present study, we first detected IAV genes in wild raccoons captured during periods other than the wintering season of migratory waterfowl and epidemic season of influenza in Japan. Viral matrix (M) and nucleoprotein (NP) genes were detected by a conventional reverse transcription-polymerase chain reaction assay from three suckling siblings and one juvenile without any noticeable clinical signs, although the NP gene could not be detected from one sibling. The sequences of M gene fragments detected from the rectal swabs of three suckling siblings were comparable with each other but different from those detected from the nasal swab of the juvenile raccoon caught from a different site. The sequences of NP gene fragments detected from two suckling siblings were also comparable. These genetic evidences suggest that IAV is maintained among raccoon populations in the northern part of Japan. Further genetic and virological investigation of IAV infection in wild raccoons is needed to better understand the IAV ecology in the field.