Interaction of spermine derivative porphyrin with DNA
Interaction of spermine derivative porphyrin with DNA
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DOI：
10.1142/s1088424618500645
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发表时间：
2018-07-01
影响因子：
1.5
通讯作者：
D'Urso, Alessandro
中科院分区：
文献类型：
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作者：
Gangemi, Chiara M. A.;D'Agostino, Bruno;D'Urso, Alessandro
卟啉类化合物由于其独特的光谱特性、迷人的光化学性质和合成的多样性而成为DNA合成的理想化合物。在氧存在下作为光敏剂的能力促进了科学界对研究卟啉与DNA的非共价结合的兴趣，以便将它们用于光动力疗法。因此，了解卟啉与DNA结合的机制有助于寻找具有特异性碱基降解活性的卟啉。本工作利用紫外-可见光谱、电子圆二色光谱和共振光散射等技术研究了精胺衍生物卟啉与三种不同的多核苷酸：ct-DNA、poly（dG-dC）（2）在B-和Z-构象下的结合模式。
Porphyrinoids are ideal compounds to be incorporated into DNA due to their peculiar spectroscopic characteristics, fascinating photochemistry and synthetic versatility. The ability to act as photosensitizers in the presence of oxygen promoted interest of the scientific community to study the non-covalent binding of porphyrins with DNA in order to use them in photodynamic therapy. Thus, the understanding of the mechanisms involved in the binding of porphyrins with DNA can help to find a porphyrin with specific base degradation activity. In this work we characterized the binding modes of spermine derivative porphyrin with three different polynucleotides: ct-DNA, poly(dG-dC)(2) in both B- and Z-conformation using several spectroscopic techniques (UV-vis, Electronic Circular Dichroism and Resonance Light Scattering).