Glyphosate based-herbicide exposure affects gut microbiota, anxiety and depression-like behaviors in mice
Glyphosate based-herbicide exposure affects gut microbiota, anxiety and depression-like behaviors in mice
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DOI：
10.1016/j.ntt.2018.04.002
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发表时间：
2018-05-01
影响因子：
2.9
通讯作者：
Bennis, Mohamed
中科院分区：
文献类型：
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作者：
Aitbali, Yassine;Ba-M'hamed, Saadia;Bennis, Mohamed
最近，许多研究已经证明了肠道微生物群（GM）改变和行为变化之间的深刻关系。草甘膦除草剂（GBH）已被证明会诱导行为障碍，并且它们可能通过改变GM来介导这种影响。本研究采用小鼠旷场实验、高架十字迷宫实验、悬尾实验和飞溅实验，研究了急性、亚慢性和慢性染毒GBH（250或500 mg/kg/d）对GM的毒性作用及其对小鼠神经行为功能的影响。然后，处死小鼠并收集肠道样品用于GM分析。亚慢性和慢性暴露于GBH诱导焦虑和抑郁样行为的增加。此外，GBH显著改变了关键微生物的相对丰度和转基因多样性方面的GM组成。事实上，它减少了更具体的，棒状杆菌，厚壁菌门，拟杆菌和乳酸杆菌在治疗mice. These数据加强了GM和GBH毒性小鼠之间的重要联系，并表明，观察到的肠道生态失调可能会增加神经行为改变的患病率。
Recently, a number of studies have demonstrated the profound relationship between gut microbiota (GM) alterations and behavioral changes. Glyphosate-based herbicides (GBH) have been shown to induce behavioral impairments, and it is possible that they mediate the effects through an altered GM. In this study, we investigated the toxic effects of GBH on GM and its subsequent effects on the neurobehavioral functions in mice following acute, subchronic and chronic exposure to 250 or 500 mg/kg/day.The effect of these acute and repeated treatments was assessed at the behavioral level using the open field, the elevated plus maze, the tail suspension and splash tests. Then, mice were sacrificed and the intestinal samples were collected for GM analysis. Subchronic and chronic exposure to GBH induced an increase of anxiety and depression-like behaviors. In addition, GBH significantly altered the GM composition in terms of relative abundance and phylogenic diversity of the key microbes. Indeed, it decreased more specifically, Corynebacterium, Firmicutes, Bacteroidetes and Lactobacillus in treated mice.These data reinforce the essential link between GM and GBH toxicity in mice and suggest that observed intestinal dysbiosis could increase the prevalence of neurobehavioral alterations.