Integrin heterodimer α9β1 is localized on the surface of porcine spermatogonial stem cells in the undifferentiated state
Integrin heterodimer α9β1 is localized on the surface of porcine spermatogonial stem cells in the undifferentiated state
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DOI：
10.1111/rda.13555
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发表时间：
2019-09-17
影响因子：
1.7
通讯作者：
Lee, Seung Tae
中科院分区：
文献类型：
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作者：
Park, Min Hee;Yun, Jung Im;Lee, Seung Tae
之前的一项研究发现，未分化的猪精原干细胞（SSCs）不粘附于腱生蛋白C，这表明整合素α（9）和β（1）亚基在猪精原干细胞表面上无活性。然而，该研究使用了没有FNIII样重复的重组腱生蛋白C。因此，本研究使用具有FNIII样重复序列的全长天然腱生蛋白C重新评估了整合素α（9）β（1）在猪SSC的质膜上积极发挥作用的存在。整合素异源二聚体的定位和功能用免疫细胞化学、附着和抗体抑制试验证实。在细胞表面具有整合素α（9）β（1）的未分化猪SSC中，与缺乏天然腱生蛋白C的细胞相比，与天然腱生蛋白C的粘附显著更高，并且与没有功能阻断相比，整合素α（9）β（1）的功能阻断显著抑制与天然腱生蛋白C的粘附。因此，我们证实了整合素α（9）和β（1）亚基在未分化状态的猪精原干细胞表面上作为活性异源二聚体起作用。
A previous study found that undifferentiated porcine spermatogonial stem cells (SSCs) did not adhere to tenascin C, indicating that the integrin alpha(9) and beta(1) subunits are inactive on the surface of porcine SSCs. However, that study used recombinant tenascin C without FNIII-like repeats. Therefore, this study re-evaluated the existence of integrin alpha(9)beta(1) actively functioning on the plasma membrane of porcine SSCs using full-length native tenascin C with FNIII-like repeats. The localization and function of the integrin heterodimer were confirmed using immunocytochemistry, attachment and antibody inhibition assays. In undifferentiated porcine SSCs with integrin alpha(9)beta(1) on the cell surface, adhesion to native tenascin C was significantly higher compared with cells lacking native tenascin C and functional blocking of integrin alpha(9)beta(1) significantly inhibited the attachment to native tenascin C compared with no functional blocking. Accordingly, we confirmed that the integrin alpha(9) and beta(1) subunits function as an active heterodimer on the surface of porcine SSCs in the undifferentiated state.