Characterization of the human leukocyte GPI-anchored glycoprotein CDw108 and its relation to other similar molecules
Characterization of the human leukocyte GPI-anchored glycoprotein CDw108 and its relation to other similar molecules
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DOI：
10.1016/s0171-2985(99)80073-4
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发表时间：
1999-06-01
期刊：
影响因子：
2.8
通讯作者：
Horejsí, V
中科院分区：
文献类型：
--
作者：
Angelisová, P;Drbal, K;Horejsí, V
CDw 108糖蛋白表达于一些白血病细胞系、红细胞和活化淋巴细胞的表面。其表面表达在各种活化刺激（PHA、PWM、Con A、PMA、抗CD 3）后迅速上调，随后逐渐降低。该分子通过糖基磷脂酰肌醇（GPI）部分锚定在膜上，其分子量为75-80 kDa，pI为5.0-5.5。内切糖苷酶F和H使其表观大小减小约10%，如通过SDS PAGE测定的。分别为15和22 kDa。它是与蛋白激酶相关的大型抗洗涤剂GPI复合物的组分。除了先前描述的CDw 108与JMH血型抗原的同一性之外，我们在这里证明了它与先前描述的由单克隆抗体H105和KS.2识别的糖蛋白的同一性，并且排除了它与另一种类似大小的GPI锚定的糖蛋白黑素转铁蛋白（gp 97）的同一性。
The CDw108 glycoprotein is expressed on the surface of some leukemic cell lines, erythrocytes and on activated lymphocytes. Its surface expression is rapidly upregulated following various activating stimuli (PHA, PWM, Con A, PMA, anti-CD3) and subsequently gradually decreases. The molecule is anchored in the membrane via glycosylphosphatidylinositol (GPI) moiety, it has molecular mass of 75-80 kDa and pI of 5.0-5.5. Endoglycosidase F and H reduce its apparent size as determined by SDS PAGE by approx. 15 and 22 kDa, respectively. It is a component of large, detergent-resistant GPI-complexes associated with protein kinases. In addition to the previously described identity of CDw108 with the JMH blood group antigen, we demonstrate here its identity to the previously described glycoprotein recognized by monoclonal antibodies H105 and KS.2, and exclude its identity with another GPI-anchored glycoprotein of similar size, melanotransferrin (gp97).