Comparative Study of the Effect of Sample Pretreatment and Extraction on the Determination of Flavonoids from Lemon (Citrus limon)
Comparative Study of the Effect of Sample Pretreatment and Extraction on the Determination of Flavonoids from Lemon (Citrus limon)
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DOI：
10.1371/journal.pone.0148056
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发表时间：
2016-01-25
期刊：
影响因子：
3.7
通讯作者：
Luque de Castro, Maria D.
中科院分区：
文献类型：
--
作者：
Ledesma-Escobar, Carlos A.;Priego-Capote, Feliciano;Luque de Castro, Maria D.
黄酮类化合物已被证明对人体健康有多种有益作用，也受到食品和制药行业的重视。柑橘类水果是类黄酮的重要来源，因此促进了对类黄酮的研究。这些研究的特点是样品预处理和提取方法之间存在差异，并且迄今为止开展的比较研究较少。目的评价样品前处理和提取方法对柠檬黄酮含量的影响。结果对摇提、超声辅助提取、微波辅助提取、过热液体提取等方法提取的新鲜、冻干、风干样品进行LC-QTOF质谱分析，利用MS/MS信息初步鉴定出32种黄酮类化合物。对新鲜和脱水样品以及不同方法提取的数据进行方差分析显示，26种和32种黄酮类化合物分别具有显著性(p
BackgroundFlavonoids have shown to exert multiple beneficial effects on human health, being also appreciated by both food and pharmaceutical industries. Citrus fruits are a key source of flavonoids, thus promoting studies to obtain them. Characteristics of these studies are the discrepancies among sample pretreatments and among extraction methods, and also the scant number of comparative studies developed so far.ObjectiveEvaluate the effect of both the sample pretreatment and the extraction method on the profile of flavonoids isolated from lemon.ResultsExtracts from fresh, lyophilized and air-dried samples obtained by shaking extraction (SE), ultrasound-assisted extraction (USAE), microwave-assisted extraction (MAE) and superheated liquid extraction (SHLE) were analyzed by LC-QTOF MS/MS, and 32 flavonoids were tentatively identified using MS/MS information. ANOVA applied to the data from fresh and dehydrated samples and from extraction by the different methods revealed that 26 and 32 flavonoids, respectively, were significant (p