Segmental structure of the Brassica napus genome based on comparative analysis with Arabidopsis thaliana
Segmental structure of the Brassica napus genome based on comparative analysis with Arabidopsis thaliana
复制标题
DOI：
10.1534/genetics.105.042093
复制
发表时间：
2005-10-01
期刊：
影响因子：
3.3
通讯作者：
Lydiate, DJ
中科院分区：
文献类型：
--
作者：
Parkin, IAP;Gulden, SM;Lydiate, DJ
根据甘蓝型油菜遗传连锁位点与拟南芥物理连锁标记的序列相似性块，将1000多个甘蓝型油菜遗传连锁的RFLP基因座定位到拟南芥基因组中的同源位置。通过这种比较分析，可以在拟南芥基因组中鉴定出至少21个保守的基因组单位，这些单位可以被复制和重排来产生今天的甘蓝型油菜基因组。在甘蓝型油菜遗传图谱中，保守区域的长度长达50 cM，相当于拟南芥基因组中9Mb的相邻序列。也有证据表明，这两个物种之间的染色体标志，特别是着丝粒区域是保守的。观察到的油菜基因组片段结构有力地表明，现存的油菜二倍体物种是从六倍体祖先进化而来的。该比较图谱有助于利用拟南芥基因组序列在更大、更难处理的油菜多倍体基因组中进行市场和候选基因鉴定。
Over 1000 genetically linked RFLP loci in Brassica napus were mapped to homologous positions in the Arabidopsis genome on the basis of sequence similarity Blocks of genetically linked loci in B. napus frequently corresponded to physically linked markers in Arabidopsis. This comparative analysis allowed the identification of a minimum of 21 conserved genomic units within the Arabidopsis genome, which call be duplicated and rearranged to generate the present-day B. napus genome. The conserved regions extended over lengths as great as 50 cM in the B. napus genetic map, equivalent to similar to 9 Mb of contiguous sequence in die Arabidopsis genome. There was also evidence for conservation of chromosome landmarks, particularly centromeric regions, between the two species. The observed segmental structure of the Brassica genome strongly suggests that the extant Brassica diploid species evolved from a hexaploid ancestor. The comparative map assists in exploiting the Arabidopsis genomic sequence for market and candidate gene identification within the larger, intractable genomes of the Brassica polyploids.