Features of disruption mutants of genes encoding for hydrophobin Vmh2 and Vmh3 in mycelial formation and resistance to environmental stress in Pleurotus ostreatus
Features of disruption mutants of genes encoding for hydrophobin Vmh2 and Vmh3 in mycelial formation and resistance to environmental stress in Pleurotus ostreatus
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DOI：
10.1093/femsle/fnad036
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发表时间：
2023-05-16
影响因子：
2.1
通讯作者：
Honda，Yoichi
中科院分区：
文献类型：
--
作者：
Han，Junxian;Kawauchi，Moriyuki;Honda，Yoichi
疏水蛋白是一种具有亲水性和疏水性的小分子分泌蛋白质，可以在空气-水界面自组装成两亲性膜，帮助真菌形成气生菌丝。在伞菌Pleurotostreatus中，已经预测了20多个假定的疏水蛋白基因。其中，两个疏水蛋白基因vmh 2和vmh 3主要在营养菌丝体中表达。本文主要研究了Vmh 2和Vmh 3在营养菌丝中的功能。通过透射电子显微镜观察菌丝体的横截面和菌丝体表面水滴消失的时间，认为Vmh 2和Vmh 3是维持菌丝体表面疏水性所必需的。Δ vmh 3和Δ vmh 2 Δ vmh 3菌株在液体培养基上的气生菌丝形成速度相对较慢，而Δ vmh 2菌株气生菌丝形成速度没有明显变化。只有Δ vmh 3和Δ vmh 2 Δ vmh 3菌株在SDS和H2 O2胁迫条件下的生长速度比野生型菌株慢。这项研究揭示了这些疏水蛋白在抗逆性中可能的独特作用。这些结果表明，伞菌，包括P. ostreatus已经进化为具有多种不同的疏水蛋白作为适应各种环境的手段。
Hydrophobins, which are small-secreted proteins with both hydrophobic and hydrophilic parts, can self-assemble into an amphiphilic film at the air-water interface, helping the fungus to form aerial hyphae. In the agaricomycetePleurotus ostreatus, more than 20 putative hydrophobin genes have been predicted. Of these, two hydrophobin genes,vmh2andvmh3, are predominantly expressed in the vegetative mycelium. In this study, we focused on the functions of Vmh2 and Vmh3 in vegetative mycelia. Based on the observation of the mycelial cross-section by transmission electron microscopy and the disappearance time of water droplets on the mycelial surface, Vmh2 and Vmh3 were considered essential for the maintenance of the surface hydrophobicity of the mycelium. The Δvmh3and Δvmh2Δvmh3strains exhibited relatively slower aerial mycelia formation on a liquid medium, and no significant alteration was observed in Δvmh2strains. Only the Δvmh3and Δvmh2Δvmh3strains grew slower than the wild-type strain under stress conditions involving SDS and H2O2on agar plates. This study revealed possible distinct roles for these hydrophobins in stress resistance. These results suggest that Agaricomycetes, includingP. ostreatus, have evolved to possess multiple different hydrophobins as a means of adapting to various environments.