Analysis of urine for its ultraviolet-absorbing constituents by high-pressure anion-exchange chromatography.
Analysis of urine for its ultraviolet-absorbing constituents by high-pressure anion-exchange chromatography.
复制标题
通过高压阴离子交换色谱法分析尿液中的紫外线吸收成分。
作者：
C. Scott
一种用于定量测定尿液和其他体液中吸收紫外线的分子成分的自动、高分辨率分析系统正在开发中。该系统由用于分离步骤的加热高压阴离子交换柱和用于检测步骤的连续流紫外分光光度计组成。分离是通过用浓度逐渐增加的醋酸缓冲液洗脱来实现的。总分析时间为40小时。显示色谱柱流出物的吸光度随时间的函数的色谱图由一个条形图记录仪和一个包含数字电压表和纸带穿孔的数据采集系统记录下来。然后，后者的输出用作数字计算机的输入，用于分析色谱图。超过140个色谱峰已从一个2毫升。尿液样本。正常尿液样品的色谱图相似。已注意到昼夜周期的变化。
An automatic, high-resolution analytic system for the quantitative determination of the ultraviolet-absorbing molecular constituents of urine and other body fluids is being developed. This system is comprised of a heated, high-pressure anion-exchange column for the separation step and a continuous-flow ultraviolet spectrophotometer for the detection step. Separation is achieved by elution with an acetate buffer of gradually increasing concentration. The total analysis time is 40 hr. Chromatograms showing the absorbance of the column effluent as a function of time are recorded by a strip-chart recorder and by a data acquisition system containing a digital voltmeter and paper tape punch. The output from the latter is then used as the input to a digital computer for analysis of the chromatogram. More than 140 chromatographic peaks have been resolved from a 2-ml. urine sample. Normal urine samples yield similar chromatograms. A variation in the diurnal cycle has been noted.