Use of cotinine immunoassay test strips for preclassifying urine samples from smokers and nonsmokers prior to analysis by LC-MS-MS
Use of cotinine immunoassay test strips for preclassifying urine samples from smokers and nonsmokers prior to analysis by LC-MS-MS
复制标题
DOI：
10.1093/jat/29.8.814
复制
发表时间：
2005-11-01
影响因子：
2.5
通讯作者：
McGuffey, JE
中科院分区：
文献类型：
--
作者：
Bernert, JT;Harmon, TL;McGuffey, JE
涉及吸烟者和非吸烟者的可替宁生物标志物测量必须适应广泛的浓度范围。因此，我们常规预分类未知样品为“高”或“低”，通过使用酶联免疫测定可替宁之前，通过串联质谱法（MS）分析。虽然这种方法是有效的，但它也是耗时和复杂的;更简单和更快的方法将是有用的。因此，作为可能的替代方案，检查了使用免疫色谱测试条（NicAlert™）进行的尿液可替宁筛选试验，随后进行数据的计算机化分析。结果表明，这种方法可以提供有用的分类效率时，使用我们的目标截止值约为20 ng/mL。在分析50个尿样与不同程度的暴露于环境烟草烟雾的非吸烟者，分类的灵敏度和特异性分别为88%和92%，相对于总可替宁浓度测量的大气压电离串联MS的测试条测量的可替宁。然而，相对较高的成本条可能是一个限制因素。
Cotinine biomarker measurements involving both smokers and nonsmokers must accommodate a broad range of concentrations. Thus, we have routinely preclassified unknown samples as being either “high” or “low” by using an enzyme-linked immunoassay for cotinine prior to analysis by tandem mass spectrometry (MS). Although this method is effective, it is also time-consuming and complex; a simpler and faster approach would be useful. Consequently, a screening assay for urine cotinine using an immunochromatographic test strip (NicAlert™) followed by a computerized analysis of the data was examined as a possible alternative. The results indicate that this approach can provide useful classification efficiency when using our target cutoff value of approximately 20 ng/mL. In the analysis of 50 urine samples from nonsmokers with varying degrees of exposure to environmental tobacco smoke, the classification sensitivity and specificity were 88% and 92%, respectively, for cotinine measured by the test strips relative to total cotinine concentrations measured by atmospheric-pressure ionization tandem MS. However, the relatively high cost of the strips may be a limiting factor.