ADF10 shapes the overall organization of apical actin filaments by promoting their turnover and ordering in pollen tubes
ADF10 shapes the overall organization of apical actin filaments by promoting their turnover and ordering in pollen tubes
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ADF10 通过促进花粉管中顶端肌动蛋白丝的周转和排序来塑造顶端肌动蛋白丝的整体组织
DOI：
10.1242/jcs.207738
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发表时间：
2017-12-01
影响因子：
4
通讯作者：
Huang, Shanjin
中科院分区：
文献类型：
--
作者：
Jiang, Yuxiang;Wang, Juan;Huang, Shanjin
在这里，我们表明，拟南芥ADF 10起着重要的作用，通过促进他们的营业额和秩序，在塑造顶端肌动蛋白丝的整体组织。ADF10在体外切断和解聚肌动蛋白丝，并分布在整个花粉管中。在adf10突变体中，顶端肌动蛋白丝的切断和单体解离事件减少，并且顶端肌动蛋白结构比野生型管进一步向管基部延伸。特别是，顶端的肌动蛋白丝，形成大角度的管生长轴的百分比高得多，在adf10花粉管，和肌动蛋白丝更随机分布，这意味着，ADF10促进其排序。与顶端肌动蛋白丝在物理上限制囊泡运动的作用一致，adf 10花粉管顶端囊泡聚集的区域扩大。在adf 10花粉管的生长区域内，小泡的向上和向后运动都发生了变化。因此，我们的研究表明，ADF 10形状顶端肌动蛋白丝的组织，以调节囊泡运输和花粉管生长。总结：ADF10是形成顶端肌动蛋白丝的整体组织的重要调节剂，并通过促进丝周转和有序化以及限制其长度来起作用。
ABSTRACT Here, we show that Arabidopsis ADF10 plays an important role in shaping the overall organization of apical actin filaments by promoting their turnover and ordering. ADF10 severs and depolymerizes actin filaments in vitro and is distributed throughout the entire pollen tube. In adf10 mutants, severing and monomer dissociation events for apical actin filaments are reduced, and the apical actin structure extends further toward the tube base than in wild-type tubes. In particular, the percentage of apical actin filaments that form large angles to the tube growth axis is much higher in adf10 pollen tubes, and the actin filaments are more randomly distributed, implying that ADF10 promotes their ordering. Consistent with the role of apical actin filaments in physically restricting the movement of vesicles, the region in which apical vesicles accumulate is enlarged at the tip of adf10 pollen tubes. Both tipward and backward movements of small vesicles are altered within the growth domain of adf10 pollen tubes. Thus, our study suggests that ADF10 shapes the organization of apical actin filaments to regulate vesicle trafficking and pollen tube growth. Summary: ADF10 is an important regulator in shaping the overall organization of apical actin filaments and acts by promoting filament turnover and ordering as well as limiting their length.