The GABAA/benzodiazepine receptor is a heterotetramer of homologous alpha and beta subunits.
The GABAA/benzodiazepine receptor is a heterotetramer of homologous alpha and beta subunits.
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GABAA/苯二氮卓受体是同源α和β亚基的异四聚体。
DOI：
10.1002/j.1460-2075.1987.tb04791.x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
E. Barnard
中科院分区：
文献类型：
--
作者：
C. Mamalaki;F. Stephenson;E. Barnard
GABAA受体已从牛大脑皮层纯化至同质。在严格的分离条件下，GABAA受体仅由α（Mr 53 000）和β（Mr 57 000）亚基组成。还原条件下SDS-PAGE凝胶的光密度扫描显示，这些亚基以1：1的比例存在。作为一个异源的四聚体α 2 β 2受体的模型提出。已经产生了针对纯化的牛GABAA受体的单克隆抗体。这些抗体之一，1A 6，显示与纯化受体的α和β亚基反应。在通过糖苷内切酶F处理去除相应多肽的碳水化合物部分后，亚基在免疫印迹中仍然是阳性的。该抗体已用于证明三种脊椎动物物种的GABAA受体之间的抗原交叉反应性。进一步提出，在α和β多肽之间存在部分氨基酸序列同源性，因此它们源自单个祖先基因。
The GABAA receptor has been purified to homogeneity from bovine cerebral cortex. Under stringent conditions of isolation, the GABAA receptor was shown to consist only of alpha (Mr 53 000) and beta (Mr 57 000) subunits. A densitometric scan of SDS‐PAGE gels under reducing conditions showed that these subunits were present in a 1:1 ratio. A model of the receptor as a heterologous tetramer alpha 2 beta 2 is proposed. Monoclonal antibodies have been raised to the purified bovine GABAA receptor. One of these antibodies, 1A6, was shown to react with both the alpha and beta subunits of the purified receptor. The subunits were still positive in immunoblots following the removal of the carbohydrate moieties of the respective polypeptides by endoglycosidase F treatment. This antibody has been employed to demonstrate antigenic cross‐reactivity between the GABAA receptors of three vertebrate species. It is further proposed that there is partial amino acid sequence homology between the alpha and beta polypeptides and hence that they are derived from a single ancestral gene.