Microbial metabolic exchange in 3D
Microbial metabolic exchange in 3D
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DOI：
10.1038/ismej.2012.155
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发表时间：
2013-04-01
期刊：
影响因子：
11
通讯作者：
Dorrestein, Pieter C.
中科院分区：
文献类型：
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作者：
Watrous, Jeramie D.;Phelan, Vanessa V.;Dorrestein, Pieter C.
单物种和多物种微生物群体在菌落发育过程中改变其周围环境的化学成分，从而影响邻近微生物的多细胞行为和种间相互作用。在这里，我们提出了一种方法，可以通过多模态成像分析创建微生物化学型的三维（3D）模型，该模型可以与菌落表型相关。这些模型是通过对生长在8 mm深琼脂上的微生物菌落的连续横截面进行基质辅助激光解吸电离飞行时间（MALDI-TOF）成像质谱（IMS）生成的，在MATLAB中注册每个连续截面的数据集以创建模型，然后将模型与菌落本身的照片叠加。作为原理证明，3D模型被用于可视化枯草芽孢杆菌和colicolor链霉菌以及白色念珠菌和铜绿假单胞菌之间微生物相互作用期间的代谢交换。由此产生的模型能够捕获琼脂培养基中分泌代谢物的深度分布，并揭示了以前使用二维MALDI-TOF IMS无法观察到的某些质量信号的特性。最重要的是，3D模型能够绘制出以前未观察到的白色念珠菌亚表面菌丝阵列中的化学分布，以及铜绿假单胞菌（一种已知可以改变白色念珠菌毒力的机会性病原体）的存在如何改变这种化学分布。结果表明，白色念珠菌的存在引发了铜绿假单胞菌鼠李糖脂产量的增加，这反过来又能够抑制室温下白色念珠菌菌落下产生的嵌入菌丝生长。ISME学报（2013）7,770 -780；doi: 10.1038 / ismej.2012.155;2013年1月3日在线发布
Mono- and multispecies microbial populations alter the chemistry of their surrounding environments during colony development thereby influencing multicellular behavior and interspecies interactions of neighboring microbes. Here we present a methodology that enables the creation of three-dimensional (3D) models of a microbial chemotype that can be correlated to the colony phenotype through multimodal imaging analysis. These models are generated by performing matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) imaging mass spectrometry (IMS) on serial cross-sections of microbial colonies grown on 8 mm deep agar, registering data sets of each serial section in MATLAB to create a model, and then superimposing the model with a photograph of the colonies themselves. As proof-of-principle, 3D models were used to visualize metabolic exchange during microbial interactions between Bacillus subtilis and Streptomyces coelicolor, as well as, Candida albicans and Pseudomonas aeruginosa. The resulting models were able to capture the depth profile of secreted metabolites within the agar medium and revealed properties of certain mass signals that were previously not observable using two-dimensional MALDI-TOF IMS. Most significantly, the 3D models were capable of mapping previously unobserved chemical distributions within the array of sub-surface hyphae of C. albicans and how this chemistry is altered by the presence of P. aeruginosa, an opportunistic pathogen known to alter virulence of C. albicans. It was determined that the presence of C. albicans triggered increased rhamnolipid production by P. aeruginosa, which in turn was capable of inhibiting embedded hyphal growth produced beneath the C. albicans colony at ambient temperature. The ISME Journal (2013) 7, 770-780; doi:10.1038/ismej.2012.155; published online 3 January 2013