Heat and nutritional shock-induced proteins of the fungus Achlya are different and under independent transcriptional control
Heat and nutritional shock-induced proteins of the fungus Achlya are different and under independent transcriptional control
复制标题
真菌 Achlya 的热和营养休克诱导的蛋白质是不同的，并且处于独立的转录控制之下
DOI：
10.1139/o84-107
复制
发表时间：
1984
影响因子：
3.3
通讯作者：
C. Braithwaite
中科院分区：
文献类型：
--
作者：
H. LéJohn;C. Braithwaite
当多核真菌Achlya klebsiana菌株1969的指数生长细胞的温度突然从24 ° C升高到37 °C（热应激）时，至少12种预先存在的蛋白质（热休克蛋白，HSP）的合成被强烈诱导，而大多数其他细胞蛋白质的合成短暂下降。热应激2-3小时后，细胞恢复并恢复正常的蛋白质合成。如果细胞在温度升高到37 °C之前首先缺乏营养（营养应激），则诱导了相同的12种热休克蛋白，但热休克诱导蛋白和非热休克蛋白的合成在热应激4小时后下降到痕量水平。 热休克蛋白的分子量分别为96 000 a、96 000 b、85 000、72 000、70 000、69 000 a、69 000 b、68 000、60 000、52 000、26 000 a和26 000 b，它们具有相似的等电点（5.8-6.2）。 营养应激细胞显示出约28种蛋白质（营养应激蛋白，NSP）的诱导合成，当它们被...
When the temperature of exponentially growing cells of the coenocytic fungus Achlya klebsiana strain 1969 was suddenly elevated from 24 to 37 °C (thermal stress), synthesis of at least 12 preexisting proteins (heat-shock proteins, HSPs) was vigorously induced while synthesis of most other cell proteins declined transiently. After 2–3 h of thermal stress, the cells recovered and resumed normal protein synthesis. If the cells were first starved of nutrients (nutritional stress) before the temperature was raised to 37 °C, the same 12 HSPs were induced, but synthesis of both heat-shock-inducible and nonheat-shock proteins declined to trace levels after 4 h of thermal stress. Molecular weights (MW) of the HSPs were approximately 96 000a, 96 000b, 85 000, 72 000, 70 000, 69 000a, 69 000b, 68 000, 60 000, 52 000, 26 000a, and 26 000b, and they had similar isoelectric points (5.8–6.2). Nutritionally stressed cells showed an induced synthesis of some 28 proteins (nutritional stress proteins, NSPs), when they were ...