THE NS-1 POLYPEPTIDE OF THE AUTONOMOUS PARVOVIRUS MVM IS A NUCLEAR PHOSPHOPROTEIN
THE NS-1 POLYPEPTIDE OF THE AUTONOMOUS PARVOVIRUS MVM IS A NUCLEAR PHOSPHOPROTEIN
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DOI：
10.1016/0168-1702(86)90003-1
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发表时间：
1986-05-01
期刊：
影响因子：
5
通讯作者：
TATTERSALL, P
中科院分区：
文献类型：
--
作者：
COTMORE, SF;TATTERSALL, P
被自主细小病毒MVM感染的细胞合成一种主要的病毒编码的非结构蛋白，其在细胞核中积累并被显著磷酸化。肽图谱分析表明，该体内产物具有与先前在MVM编码mRNA的体外翻译产物中鉴定的NS-1蛋白相同的一级序列，因此是最大信使RNA种类的产物，剪接的4.8 kb（R1）转录物。在体内NS-1以两种主要形式存在，磷酸化的84-85 kDa种类和未磷酸化或磷酸化差的83 kDa形式。这两种形式都优先使用富集复制复合物的提取程序从细胞核中提取。
Cells infected with the autonomous parvovirus MVM synthesize a major virally coded non-structural protein which accumulates in the nucleus and is phosphorylated to a significant extent. Peptide map analysis shows that this in vivo product has the same primary sequence as the NS-1 protein previously identified in the in vitro translation products of MVM encoded mRNA, and as such is the product of the largest messenger RNA species, a spliced 4.8 kb (R1) transcript. In vivo NS-1 exists in two predominant forms, a phosphorylated 84-85 kDa species and an unphosphorylated or poorly phosphorylated 83 kDa form. Both forms are preferentially extracted from the nucleus using an extraction procedure which enriches for replication complexes.