Qualitative determination of volatile compounds and quantitative evaluation of safranal and 4-hydroxy-2,6,6-trimethyl-1-cyclohexene-1-carboxaldehyde (HTCC) in Greek saffron
Qualitative determination of volatile compounds and quantitative evaluation of safranal and 4-hydroxy-2,6,6-trimethyl-1-cyclohexene-1-carboxaldehyde (HTCC) in Greek saffron
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DOI：
10.1021/jf049808j
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发表时间：
2004-07-14
影响因子：
6.1
通讯作者：
Polissiou, MG
中科院分区：
文献类型：
--
作者：
Kanakis, CD;Daferera, DJ;Polissiou, MG
Safranal（2,6,6-三甲基-1,3-环己二烯-1-甲醛）是藏红花精油的主要成分。它是通过微量同步水力蒸馏萃取 (MSDE) 和超声辅助萃取 (USE) 获得的，这是一种温和的方法。 4-羟基-2,6,6-三甲基-1-环己烯-1-甲醛 (HTCC) 是藏花醛的前体，仅通过使用才能获得大量。首次在藏红花中发现了 5 种 C-13-降异戊二烯类化合物。使用气相色谱技术对希腊藏红花样品中的藏红花醛和 HTCC 进行定量。通过 MSDE 分离的藏红花醛量范围为 288.1 至 687.9 毫克/100 克藏红花，而在 USE 藏红花和 HTCC 的情况下，分离的藏红花醛含量范围分别为 40.7 至 647.7 毫克/100 克藏红花和 41.7 至 397.7 毫克/100 克藏红花。还测试了冷冻干燥作为替代干燥方法。在 4 摄氏度下储存多年后，番红花醛的数量基本保持不变，而 HTCC 的数量在同一时期有所下降。
Safranal (2,6,6-trimethyl-1,3-cyclohexadiene-1-carboxaldehyde) is the main component of saffron's essential oil. It was obtained using microsimultaneous hydro, distillation-extraction (MSDE) and by ultrasound-assisted extraction (USE), which is a mild method. 4-Hydroxy-2,6,6-trimethyl-1-cyclohexene-1-carboxaldehyde (HTCC) is a precursor of safranal and was obtained in considerable amounts only by USE. Five C-13-norisoprenoids were found in saffron for the first time. Using a gas chromatography technique, safranal and HTCC were quantified from Greek saffron samples. The quantity of safranal isolated by MSDE ranged between 288.1 and 687.9 mg/100 g of saffron, whereas in the case of USE safranal and HTCC ranged between 40.7 and 647.7 mg/100 g of saffron and between 41.7 and 397.7 mg/100 g of saffron, respectively. Freeze-drying was also tested as an alternative drying method. Over years of storage at 4 degreesC the quantity of safranal remained mostly constant while the quantity of HTCC decreased over the same periods.