CORRELATION OF DNA FLOW CYTOMETRIC RESULTS AND OTHER PROGNOSTIC FACTORS IN PRIMARY BREAST-CANCER
CORRELATION OF DNA FLOW CYTOMETRIC RESULTS AND OTHER PROGNOSTIC FACTORS IN PRIMARY BREAST-CANCER
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DOI：
10.1002/ijc.2910410608
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发表时间：
1988-06-15
影响因子：
6.4
通讯作者：
GOERTTLER, K
中科院分区：
文献类型：
--
作者：
FEICHTER, GE;MUELLER, A;GOERTTLER, K
应用DNA流式细胞术（FCM）检测了300例原发性乳腺癌的S期细胞百分率和DNA倍体。并与患者年龄、绝经状况、肿瘤大小、淋巴结转移、组织学类型、分级、雌激素受体（ER）和孕激素受体（PR）浓度等进行比较。DNA二倍体分布的G 0/1峰被发现在37.6%的情况下。S期组分的平均百分比为4.83。DNA非整倍体肿瘤的S期比例（6.12%）显著高于DNA二倍体肿瘤（2.66%）。DNA测量数据（DNA倍体和S期分数）与组织学分级以及ER和PR控制之间也存在显著相关性，但DNA倍体、S期分数、肿瘤大小（T）与腋窝淋巴结转移证据之间无相关性。DNA-FCM除了组织病理学检查外，还提供了肿瘤的生物学特征。该方法可用作常规程序，因为结果的可靠性和重现性以及测量所需的时间短。
The percentage of cells in S-phase and DNA-ploidy have been measured in 300 primary mammary carcinomas by means of DNA-flow cytometry (FCM). The data were compared with the age and menopausal status of the patients as well as with the size, regional lymph-node involvement, histologic type, grade and concentration of estrogen (ER) and progesterone (PR) receptors of the tumors. A DNA-diploid distribution of the G0/1-peak was found in 37.6% of the cases. The mean percentage of S-phase fractions was 4.83. DNA-aneuploid tumors had significantly higher amounts of S-phase fractions (6.12%) than DNA-diploid tumors (2.66%). There was also a significant correlation between the DNA measurement data (DNA-ploidy and S-phase fractions) and histologic grade, as well as the control of ER and PR, but not between DNA-ploidy, S-phase fractions, tumor size (T) and evidence of axillary lymph-node metastases. DNA-FCM gives a biological characterization of the tumor in addition to the histopathologic examination. The method can be used as a routine procedure because of the reliability and reproducibility of the results as well as the short time needed for the measurements.