Dexamethasone in vivo raises surfactant protein B mRNA in alveolar and bronchiolar epithelium.
Dexamethasone in vivo raises surfactant protein B mRNA in alveolar and bronchiolar epithelium.
复制标题
体内地塞米松可提高肺泡和细支气管上皮中表面活性蛋白 B mRNA 的水平。
DOI：
10.1152/ajplung.1991.260.2.l146
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Floros,J
中科院分区：
文献类型：
--
作者：
Phelps,DS;Floros,J
糖皮质激素通过增加II型细胞表面活性物质的产生，部分地提高了胎肺的成熟率。我们已经研究了在体内地塞米松治疗的疏水表面活性剂蛋白，SP-B，在大鼠肺的mRNA水平的影响，并比较了类似的测量SP-A mRNA的数据。对已知胎龄和出生后胎龄的大鼠腹腔注射地塞米松（2 mg/kg），24 h后处死。用大鼠SP-A和SP-B cDNA探针通过RNA印迹法测定肺中SP-B mRNA水平。SP-B mRNA水平增加了5.2倍，当163对所有年龄的动物进行了检查。胎儿（107对）和出生后动物（56对）的SP-B mRNA分别增加了8.4倍和2.1倍（P <0.0001）。胎肺中的高水平刺激主要是由于妊娠第18天和第19天的高增加，此时对照水平非常低。在整个人口中没有可检测到的性别差异。组织原位杂交显示，II型细胞，以及一些细支气管细胞，地塞米松治疗的整个生命。SP-B在细支气管上皮中的存在和激素反应性的意义和原因是有趣的，值得进一步研究。
Glucocorticoids enhance the rate of maturation of the fetal lung, in part, by increasing production of surfactant by type II cells. We have examined the effect of in vivo dexamethasone treatment on mRNA levels for the hydrophobic surfactant protein, SP-B, in rat lung and compared the data to similar measurements of SP-A mRNA. Rats of known gestational and postnatal ages were injected intraperitoneally with dexamethasone (2 mg/kg) and killed after 24 h. SP-B mRNA levels in the lungs were determined by RNA blotting using rat SP-A and SP-B cDNA probes. SP-B mRNA levels increased 5.2-fold when 163 pairs of animals of all ages were examined. SP-B mRNA increases in fetuses (107 pairs) and postnatal animals (56 pairs) were 8.4- and 2.1-fold, respectively (P less than 0.0001). The high levels of stimulation in fetal lungs were largely due to high increases on gestational days 18 and 19 when the control levels are very low. There were no detectable gender differences in the population as a whole. Tissue in situ hybridization showed that type II cells, as well as some bronchiolar cells, responded to dexamethasone treatment throughout life. The significance and the reasons for the presence and hormonal responsiveness of SP-B in the bronchiolar epithelium are intriguing and warrant further investigation.