UTMD promoted local delivery of miR-34a-mimic for ovarian cancer therapy.
UTMD promoted local delivery of miR-34a-mimic for ovarian cancer therapy.
复制标题
DOI：
10.1080/10717544.2021.1955041
复制
发表时间：
2021-12
期刊：
影响因子：
6
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Li Y;Du M;Fang J;Zhou J;Chen Z
MicroRNA-mediated gene therapy is emerging as a promising method for the treatment of ovarian cancer, but the development of miRNA mimic delivery vectors is still in its infancy, where the safety and efficacy of miR-34a-mimic remain unknown. Ultrasound-targeted microbubble destruction (UTMD) can be an effective and minimally invasive tool for the delivery of miR-34a-mimic in vitro and in vivo. Here, we describe a high-efficiency gene delivery strategy by using miR-34a-mimic loaded folate modified microbubbles (miR-34a-FM) with a portable ultrasonic irradiation system. Ultrasonic parameters, including acoustic intensity (AI), exposure time (ET) and duty cycle (DC), were optimized and the optimal acoustic condition (1.0 W/cm2, 20 s, and 15% DC) was used to deliver miRNA-34a into cells in vitro. MiR-34a mimic was successfully introduced into the cytoplasm and was found to inhibit proliferation and induce apoptosis of SK-OV-3 cells. Next, miR-34a-mimic was delivered to tumor tissue via UTMD, inhibiting tumor growth and prolonging the survival time of mice. In summary, UTMD-mediated miR-34a-mimic delivery has potential application in the clinical treatment of ovarian cancer.
登录
查看更多内容
利用连续低能量声波探测脉冲进行高效分子输送。
影响因子：
12.4
作者：
Song KH;Fan AC;Brlansky JT;Trudeau T;Gutierrez-Hartmann A;Calvisi ML;Borden MA
通讯作者：
Borden MA
DOI：
10.1016/j.jconrel.2015.12.001
发表时间：
2016-02-10
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Shapiro G;Wong AW;Bez M;Yang F;Tam S;Even L;Sheyn D;Ben-David S;Tawackoli W;Pelled G;Ferrara KW;Gazit D
通讯作者：
Gazit D
影响因子：
10.8
作者：
De Cock, Ine;Zagato, Elisa;Lentacker, Ine
通讯作者：
Lentacker, Ine
DOI：
10.1073/pnas.1606915113
发表时间：
2016-09-06
影响因子：
11.1
作者：
Helfield, Brandon;Chen, Xucai;Villanueva, Flordeliza S.
通讯作者：
Villanueva, Flordeliza S.
影响因子：
50.3
作者：
Jackstadt, Rene;van Hooff, Sander R.;Sansom, Owen J.
通讯作者：
Sansom, Owen J.