Evidence of Bacterial Biofilms among Infected and Hypertrophied Tonsils in Correlation with the Microbiology, Histopathology, and Clinical Symptoms of Tonsillar Diseases.
Evidence of Bacterial Biofilms among Infected and Hypertrophied Tonsils in Correlation with the Microbiology, Histopathology, and Clinical Symptoms of Tonsillar Diseases.
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DOI：
10.1155/2013/408238
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发表时间：
2013
影响因子：
--
通讯作者：
Abdulla MA
中科院分区：
文献类型：
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作者：
Alasil SM;Omar R;Ismail S;Yusof MY;Dhabaan GN;Abdulla MA
由于细菌通过形成生物膜而持续存在，扁桃体疾病对抗生素的耐药性越来越强。因此，了解这些疾病的微生物学和病理生理学是管理生物膜相关感染的重要一步。我们分离了微生物，评估了它们的抗菌敏感性，并检测了扁桃体标本中存在与扁桃体疾病临床表现相关的细菌生物膜。因此，从马来亚大学医学中心接受扁桃体切除术的70名患者身上共收集了140个腭扁桃体。检出最多的是金黄色葡萄球菌（39.65%），其次是流感嗜血杆菌（18.53%）。除复方新诺明外，其余抗菌药物均有较高的敏感性。60%的患者检测到细菌生物膜，相当大比例的患者表现为感染而不是阻塞。此外，打鼾、呼吸暂停、鼻塞和扁桃体肥大等临床症状与扁桃体的微生物学有关，特别是与生物膜的存在有关。总之，扁桃体中生物膜与临床症状相关的证据解释了扁桃体疾病的顽固性，并强调了生物膜早期检测和预防对更好地治疗生物膜相关感染的重要性。
Diseases of the tonsils are becoming more resistant to antibiotics due to the persistence of bacteria through the formation of biofilms. Therefore, understanding the microbiology and pathophysiology of such diseases represent an important step in the management of biofilm-related infections. We have isolated the microorganisms, evaluated their antimicrobial susceptibility, and detected the presence of bacterial biofilms in tonsillar specimens in correlation with the clinical manifestations of tonsillar diseases. Therefore, a total of 140 palatine tonsils were collected from 70 patients undergoing tonsillectomy at University Malaya Medical Centre. The most recovered isolate was Staphylococcus aureus (39.65%) followed by Haemophilus influenzae (18.53%). There was high susceptibility against all selected antibiotics except for cotrimoxazole. Bacterial biofilms were detected in 60% of patients and a significant percentage of patients demonstrated infection manifestation rather than obstruction. In addition, an association between clinical symptoms like snore, apnea, nasal obstruction, and tonsillar hypertrophy was found to be related to the microbiology of tonsils particularly to the presence of biofilms. In conclusion, evidence of biofilms in tonsils in correlation with the demonstrated clinical symptoms explains the recalcitrant nature of tonsillar diseases and highlights the importance of biofilm's early detection and prevention towards better therapeutic management of biofilm-related infections.