Hemoglobin Promotes Staphylococcus aureus Nasal Colonization
Hemoglobin Promotes Staphylococcus aureus Nasal Colonization
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DOI：
10.1371/journal.ppat.1002104
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发表时间：
2011-07-01
期刊：
影响因子：
6.7
通讯作者：
Boles, Blaise R.
中科院分区：
文献类型：
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作者：
Pynnonen, Melissa;Stephenson, Rachel E.;Boles, Blaise R.
金黄色葡萄球菌鼻腔定植是社区和医院感染的重要危险因素。尽管S.金黄色葡萄球菌对人类健康的影响，影响鼻腔定植的分子机制和宿主因素尚不清楚。为了确定导致鼻腔定植的宿主因素，我们收集了人类鼻腔分泌物并分析了它们促进S。金黄色葡萄球菌表面定殖。一些个体产生的分泌物具有显著促进S.金黄色葡萄球菌表面定殖。鼻分泌物经蛋白酶预处理后不再促进S。金黄色葡萄球菌表面定植，表明蛋白质因子的参与。对分泌物的主要蛋白质成分进行了鉴定，随后的分析表明血红蛋白具有促进S。金黄色葡萄球菌表面定殖。鼻分泌物中血红蛋白的免疫沉淀导致S。金黄色葡萄球菌表面定殖。此外，外源添加的血红蛋白显着降低了啮齿动物模型中鼻定植所需的接种物。最后，我们发现血红蛋白阻止了agr群体感应系统的表达，并且agr效应分子RNAIII的异常组成型表达导致了S.金黄色。总的来说，我们的研究结果表明，鼻分泌物中血红蛋白的存在有助于S。金黄色葡萄球菌鼻定植。
Staphylococcus aureus nasal colonization is an important risk factor for community and nosocomial infection. Despite the importance of S. aureus to human health, molecular mechanisms and host factors influencing nasal colonization are not well understood. To identify host factors contributing to nasal colonization, we collected human nasal secretions and analyzed their ability to promote S. aureus surface colonization. Some individuals produced secretions possessing the ability to significantly promote S. aureus surface colonization. Nasal secretions pretreated with protease no longer promoted S. aureus surface colonization, suggesting the involvement of protein factors. The major protein components of secretions were identified and subsequent analysis revealed that hemoglobin possessed the ability to promote S. aureus surface colonization. Immunoprecipitation of hemoglobin from nasal secretions resulted in reduced S. aureus surface colonization. Furthermore, exogenously added hemoglobin significantly decreased the inoculum necessary for nasal colonization in a rodent model. Finally, we found that hemoglobin prevented expression of the agr quorum sensing system and that aberrant constitutive expression of the agr effector molecule, RNAIII, resulted in reduced nasal colonization of S. aureus. Collectively our results suggest that the presence of hemoglobin in nasal secretions contributes to S. aureus nasal colonization.