Do wild fish species contribute to the transmission of koi herpesvirus to carp in hatchery ponds?
Do wild fish species contribute to the transmission of koi herpesvirus to carp in hatchery ponds?
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DOI：
10.1111/jfd.12016
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发表时间：
2013-05-01
影响因子：
2.5
通讯作者：
Steinhagen, D.
中科院分区：
文献类型：
--
作者：
Fabian, M.;Baumer, A.;Steinhagen, D.
锦鲤疱疹病毒（KHV）自1998年发现以来已在全球范围内传播，并导致锦鲤和鲤鱼种群的疾病和死亡，对鲤鱼生产业产生了很大影响。已进行了许多调查，以审查传播途径并查明可能的传播媒介。然而，这些答案提出了许多新的问题。在本研究中，不同的野生鱼类从鲤鱼池塘与历史的KHV感染的敏感性进行了检查。在这些鱼的组织中，确定了病毒载量，并测试了是否可以通过应激诱导病毒的释放，然后病毒可以转移到幼稚鲤鱼。野生鱼收集鲤鱼池塘在急性爆发的病毒引起的死亡率在夏季和池塘放养鲤鱼携带潜在的KHV感染。从这些池塘，野生鱼收集在秋季或春季的收获过程中，当池塘排水。我们发现，无论季节，温度变化，年龄和感染状况的鲤鱼股票，野生鱼鲤鱼池及其出口可以检测到阳性的KHV基因组与低流行率和病毒载量的实时PCR。此外，病毒转移到幼稚鲤鱼后，观察到一段时间的同居。因此，鲤科和非鲤科野生鱼类可被视为池塘鲤鱼养殖场的流行病学风险。
The koi herpesvirus (KHV) has spread worldwide since its discovery in 1998 and causes disease and mortality in koi and common carp populations with a high impact on the carp production industry. Many investigations have been conducted to examine ways of distribution and to identify possible transmission vectors. The answers, however, raise many new questions. In the present study, different wild fish species taken from carp ponds with a history of KHV infection were examined for their susceptibility to the virus. In the tissue of these fish, the virus load was determined and it was tested whether a release of the virus could be induced by stress and the virus then could be transferred to naive carp. Wild fish were gathered from carp ponds during acute outbreaks of virus-induced mortality in summer and from ponds stocked with carp carrying a latent KHV infection. From these ponds, wild fish were collected during the harvesting process in autumn or spring when the ponds were drained. We found that regardless of season, temperature variation, age and infection status of the carp stock, wild fish from carp ponds and its outlets could be tested positive for the KHV genome using real-time PCR with a low prevalence and virus load. Furthermore, virus transfer to naive carp was observed after a period of cohabitation. Cyprinid and non-cyprinid wild fish can therefore be considered as an epidemiological risk for pond carp farms.