Limitations of Folin assays of foliar phenolics in ecological studies
Limitations of Folin assays of foliar phenolics in ecological studies
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DOI：
10.1023/a:1010306103643
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发表时间：
2001-04-01
影响因子：
2.3
通讯作者：
Schultz, JC
中科院分区：
文献类型：
--
作者：
Appel, HM;Govenor, HL;Schultz, JC
我们研究了广泛使用的Folin-Denis测定纯化单宁IG木本植物物种和三个商业多酚制剂经常用作标准的反应，这些化学混合物的反应显着不同的来源（树种，商业制剂）和采样日期，即使混合物中含有相同的总干重的单宁。.对商业标准的反应通常与对实际植物单宁的反应不同，产生的估计值与实际浓度相差两倍。多酚成分的物种和季节差异显然是造成这些可变结果的原因。依赖于氧化还原反应的试剂，如Folin-Denis，在比较不同物种或来自不同日期的样品时，不能产生可靠的酚类化合物的绝对或相对定量，并且使用商业标准品不能解决这个问题。
We examined the response of the widely used Folin-Denis assay to purified tannins from IG woody plant species and to three commercial polyphenol preparations often used as standards, The reagent's response to these chemical mixtures differed significantly among sources (tree species, commercial preparations) and sampling dates, even though the mixtures contained the same total dry weight of tannins. . Response to commercial standards usually did not resemble response to actual plant tannin and produced estimates that differed from actual concentrations by as much as twofold. Species-based and seasonal differences in polyphenol composition are evidently responsible For these variable results. Reagents that depend on redox reactions, such as the Folin-Denis, do not produce reliable absolute or relative quantification of phenolics when different species or samples from different dates are compared, and use of commercial standards does not resolve this problem.