Fabrication of immunosensor microwell arrays from gold compact discs for detection of cancer biomarker proteins.
Fabrication of immunosensor microwell arrays from gold compact discs for detection of cancer biomarker proteins.
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DOI：
10.1039/c1lc20833k
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发表时间：
2012-01-21
期刊：
影响因子：
6.1
通讯作者：
Rusling JF
中科院分区：
文献类型：
--
作者：
Tang CK;Vaze A;Rusling JF
报道了一种简单的方法，以每个芯片不到0.2美元的价格从金光盘（CD）中制造具有围绕8个电极的微孔的金阵列。这些一次性金CD阵列芯片与微流体的集成提供了廉价的免疫阵列，用于以高灵敏度快速测量癌症生物标志物蛋白。金CD传感器阵列的制造使用热转移的激光喷墨墨粉从计算机打印的图案，然后选择性化学蚀刻。传感器元件具有0.42mm2的电化学可寻址表面积，RSD <2%。对于概念验证应用，将阵列集成到一个简单的微流体装置中，用于稀释血清中癌症生物标志物白细胞介素-6（IL-6）的电化学检测。将IL-6的捕获抗体化学连接到电极阵列上，并开发了夹心免疫测定方案。具有聚合辣根过氧化物酶标记的生物素化检测抗体用于信号放大。稀释血清中IL-6的检测限非常低，为10 fg mL −1（385 aM），IL-6浓度的对数在10至1300 fg mL −1范围内呈线性响应。这些易于制造的、超灵敏的微流体免疫传感器应该很容易适用于癌症诊断中多种生物标志物的灵敏检测。
A simple method is reported to fabricate gold arrays featuring microwells surrounding 8-electrodes from gold compact discs (CDs) for less than $0.2 per chip. Integration of these disposable gold CD array chips with microfluidics provided inexpensive immunoarrays that were used to measure a cancer biomarker protein quickly at high sensitivity. The gold CD sensor arrays were fabricated using thermal transfer of laserjet toner from a computer-printed pattern followed by selective chemical etching. Sensor elements had an electrochemically addressable surface area of 0.42 mm2 with RSD <2%. For a proof-of-concept application, the arrays were integrated into a simple microfluidic device for electrochemical detection of cancer biomarker interleukin-6 (IL-6) in diluted serum. Capture antibodies of IL-6 were chemically linked onto the electrode arrays and a sandwich immunoassay protocol was developed. A biotinylated detection antibody with polymerized horseradish peroxidase labels was used for signal amplification. The detection limit of IL-6 in diluted serum was remarkably low at 10 fg mL −1 (385 aM) with a linear response with log of IL-6 concentration from 10 to 1300 fg mL −1. These easily fabricated, ultrasensitive, microfluidic immunosensors should be readily adapted for sensitive detection of multiple biomarkers for cancer diagnostics.