Plum germplasm in Croatia and neighboring countries assessed by microsatellites and DUS descriptors
Plum germplasm in Croatia and neighboring countries assessed by microsatellites and DUS descriptors
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通过微卫星和 DUS 描述符评估克罗地亚及其邻国的李子种质
DOI：
10.1007/s11295-014-0721-5
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发表时间：
2014
影响因子：
2.4
通讯作者：
F. Gaši
中科院分区：
文献类型：
--
作者：
D. H. Kazija;T. Jelačić;P. Vujević;B. Milinović;D. Čiček;A. Biško;I. Pejić;S. Šimon;M. Žulj Mihaljević;M. Pecina;D. Nikolić;J. Grahić;P. Drkenda;F. Gaši
在上世纪的某个时期，前南斯拉夫（其中包括波斯尼亚和黑塞哥维那、克罗地亚和塞尔维亚）是世界上最大的李子生产国。该地区仍在种植的传统李子品种是多种李子的混合物，包括：欧洲李子 (Prunus DomesticaL.)、紫李子 (Prunus insititiavar. syriaca(Borkh.) Koehne) 和西李子 (P. insititiaL.)。利用这种李子种质的基本问题，无论是用于栽培还是育种目的，都是缺乏可靠的果树学数据或参考存储库，无法对品种进行积极的鉴定。在这项研究中，使用微卫星标记和独特性、均匀性和稳定性 (DUS) 李子描述符对 62 个李子种质（42 个传统克罗地亚种质、6 个从塞尔维亚和波斯尼亚收集的著名传统种质以及 14 个国际参考品种）进行了评估。 9 个引物对扩增了 168 个不同的等位基因，或者每个基因座平均扩增了 18.7 个等位基因。通过Fst（Fst = 0.022；P< 0.0001）、分子方差分析（AMOVA；fCT= 0.054；P< 0.05）检测到传统李子品种与国际参考品种之间存在显着差异，随后通过因子对应分析（FCA）证实。贝叶斯方法能够对米拉贝尔、西洋李和欧洲李子基因型进行分类。基于 22 个形态性状的主成分分析成功地将米拉贝尔种质与欧洲李子和西洋李种质区分开来，但观察到的形态性状与分子数据之间普遍缺乏相关性。这项研究的结果表明，传统的克罗地亚种质代表了一种多样化且未充分利用的植物遗传材料，应该予以保护。
At a certain period during the last century, former Yugoslavia (which among others used to include Bosnia and Herzegovina, Croatia, and Serbia) was the biggest producer of plums in the world. Traditional plum cultivars, still grown in this region, represent a mixture of several species including: European plums (Prunus domesticaL.), mirabelles (Prunus insititiavar. syriaca(Borkh.) Koehne), and damsons (P. insititiaL.). The basic problem with the utilization of this plum germplasm, either for cultivation or breeding purposes, is a lack of reliable pomology data or reference repositories that would enable positive identification of cultivars. In this study, 62 plum accessions (42 traditional Croatian accessions, six well-known traditional accessions collected from Serbia and Bosnia, and 14 international, reference cultivars) were assessed using microsatellite markers and distinctness, uniformity, and stability (DUS) plum descriptors. Nine primer pairs amplified 168 distinct alleles, or on average 18.7 alleles per locus. A significant differentiation between the traditional plum cultivars and international reference cultivars, was detected through Fst (Fst = 0.022;P< 0.0001), analyses of molecular variance (AMOVA;fCT= 0.054;P< 0.05) and later confirmed by a factorial correspondence analysis (FCA). Bayesian method enabled the classification of mirabelle, damson, and European plum genotypes. Principal component analyses, based on 22 morphologic traits, managed to separate mirabelle accession from the European plum and damson accessions, but there was a general lack of correlation between the observed morphologic traits and the molecular data. Results of this study indicate that traditional Croatian accessions represent a diverse and underutilized plant genetic material, which should be conserved.