Therapeutic effects of polysaccharides from Anoectochilus roxburghii on type II collagen-induced arthritis in rats
Therapeutic effects of polysaccharides from Anoectochilus roxburghii on type II collagen-induced arthritis in rats
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金线莲多糖对大鼠II型胶原性关节炎的治疗作用
DOI：
10.1016/j.ijbiomac.2018.11.015
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发表时间：
2019-02-01
影响因子：
8.2
通讯作者：
Zheng, Chengjian
中科院分区：
文献类型：
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作者：
Guo, Yaoli;Ye, Qi;Zheng, Chengjian
刺梨是一种著名的中草药，常用于治疗肝病、糖尿病和类风湿性关节炎。本研究采用ⅱ型胶原诱导关节炎（CIA）大鼠模型，探讨刺梨多糖（ARP）的抗类风湿关节炎作用。采用醇沉法制备了ARP，并采用化学、色谱和光谱学相结合的方法对其进行了结构表征。高效粒径排除色谱-多角度激光散射-折射率（HPSEC-MALLS-RI）分析表明，ARP包含两个峰，主峰的重量-平均分子量（Mw）为5.90 kDa，相对含量为98.2%。药理结果显示，ARP能显著降低CIA大鼠关节炎指数，改善炎症细胞浸润和滑膜组织破坏。此外，ARP还具有显著的NO生成抑制作用和抗氧化活性。进一步的抗炎机制研究表明，ARP通过抑制I κ B和p65的磷酸化，显著抑制核因子κ B （nf - κ B）通路的激活，从而下调lps刺激的RAW 264.7细胞中IL-1 β和IL-6的mRNA表达。这些发现表明，ARP在开发治疗类风湿性关节炎的功能性食品和膳食补充剂方面具有很大的潜力。(C) 2018年Elsevier B.V.出版
Anoectochilus roxburghii, a famous Chinese herbal medicine, has been commonly used for the treatment of liver disease, diabetes, and rheumatoid arthritis. Our study aimed to investigate the anti-rheumatoid arthritis effects of A. roxburghii polysaccharides (ARP), using the rat's model of type II collagen-induced arthritis (CIA). ARP was prepared by alcohol sedimentation and structurally characterized based on combined chemical, chromatographic and spectroscopic methods. High Performance Size Exclusion Chromatography-Multiangle Laser Light Scattering-Refrative Index (HPSEC-MALLS-RI) analysis revealed that ARP includes two peaks, and the weight-average molecular weight (Mw) of the principal one was estimated as 5.90 kDa with a relative content of 98.2%. Pharmacological results exhibited that ARP significantly decreased the arthritis index and ameliorated the inflammatory cell infiltration and the synovial tissue destruction in CIA rats. Additionally, ARP possessed significant NO production inhibitory effects and antioxidant activity. Further anti-inflammatory mechanism investigations indicated that ARP significantly inhibited the activation of nuclear factor kappa B (NF-kappa B) pathway by suppressing the phosphorylation of I kappa B and p65, which subsequently down-regulated the mRNA expressions of IL-1 beta and IL-6 in LPS-stimulated RAW 264.7 cells. These findings suggested that ARP has great potential in the development of functional foods and dietary supplements for the treatment of rheumatoid arthritis. (C) 2018 Published by Elsevier B.V.