Effects of estrogens on proliferation and differentiation of neural stem/progenitor cells
Effects of estrogens on proliferation and differentiation of neural stem/progenitor cells
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DOI：
10.2220/biomedres.29.163
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发表时间：
2008-07-01
影响因子：
1.2
通讯作者：
Furukawa, Yoshiko
中科院分区：
文献类型：
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作者：
Okada, Makiko;Murase, Koichi;Furukawa, Yoshiko
我们研究了雌性激素 17 β-雌二醇 (E2) 和激素模拟双酚 A (BPA) 对从胚胎第 15 天大鼠端脑培养的大鼠神经干/祖细胞 (NS/PC) 增殖和分化的影响。碱性成纤维细胞生长因子 (FGF-2) 是早期生成的 NS/PC 的有效有丝分裂原，用于 NS/PC 的体外增殖。单独向 NS/PC 施用 E2 或 BPA 在不存在 FGF-2 的情况下刺激其增殖，但在 FGF-2 存在的情况下则不刺激它们的增殖。 E2或BPA处理增加了NS/PC产生的少突胶质细胞与总细胞的比例；然而，当用血小板衍生生长因子（PDGF）（一种少突胶质细胞前体细胞有丝分裂原）或神经营养蛋白-3（一种神经胶质祖细胞寡生成因子）刺激细胞时，这一比例并没有改变。这些结果表明，当细胞在神经发生的早期阶段缺乏有丝分裂原或分化因子时，雌激素会影响 NS/PC 的命运。
We investigated the effect of the female hormone 17 beta-estradiol (E2) and the hormone mimic bisphenol A (BPA) on the proliferation and differentiation of rat neural stem/progenitors cells (NS/ PCs) cultured from the telencephalon of embryonic day-15 rats. Basic fibroblast growth factor (FGF-2) is a potent mitogen of early generated NS/PCs, and is used for the proliferation of NS/ PCs in vitro. Administration of E2 or BPA alone to the NS/PCs stimulated their proliferation in the absence but not in the presence of FGF-2. E2- or BPA-treatment increased the ratio of the oligodendrocytes generated from the NS/PCs to total cells; however, this ratio did not change when the cells were stimulated with platelet-derived growth factor (PDGF), a mitogen for oligodendrocyte precursors, or with neurotrophin-3, an oligogenic factor for glial progenitor cells. These results suggest that estrogens would influence the fate of NS/PCs when the cells are poorly supplied with mitogens or differentiation factors during the early stages of neurogenesis.