Endoglin is necessary for angiogenesis in human ovarian carcinoma-derived primary endothelial cells
Endoglin is necessary for angiogenesis in human ovarian carcinoma-derived primary endothelial cells
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内皮糖蛋白对于人卵巢癌来源的原代内皮细胞的血管生成是必需的
DOI：
10.4161/cbt.25940
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发表时间：
2013-08
影响因子：
3.6
通讯作者：
Liang, Zhi-Qing
中科院分区：
文献类型：
--
作者：
Zhao, Li-Mei;Zhao, Xi-Long;Shen, Jun-Jie;Xie, Yao;Cao, Li-Li;Chen, Zhen-Bo;Luo, Yan-Mei;Bao, Bi-Hui;Liang, Zhi-Qing
内皮糖蛋白（CD 105，END）在增殖的内皮细胞中上调，提示潜在的治疗特性。然而，尚不清楚内皮糖蛋白是否介导了增强的增殖速率或可能作为负反馈环的一部分被上调。为了深入了解内皮糖蛋白的环境依赖性和细胞类型依赖性调节作用，我们研究了其在人卵巢癌衍生的内皮细胞（ODMECs）中的作用特性。我们从上皮性卵巢癌组织中分离并培养了原代ODMECs。ODMECs具有更高的endoglin和VEGFR-2表达，并且在体外也表现出增强的血管样结构的自发形成。靶向内皮糖蛋白的siRNA转染ODMECs细胞导致增殖和管形成的减少。这些结果表明，一个子集的ODMECs显示异常的血管生成特性，这种表型被阻断内皮糖蛋白水平的降低，这表明内皮糖蛋白是必不可少的刺激血管生成，和靶向它可能是一个有吸引力的方法，抗血管生成治疗卵巢癌。
Endoglin (CD105, END) is upregulated in proliferating endothelial cells, suggesting potential therapeutic properties. However, it is not clear whether endoglin mediates an enhanced proliferative rate or may be upregulated as part of a negative feedback loop. To gain insights into context-dependent and cell type-dependent regulatory effects of endoglin, we studied its role properties in human ovarian carcinoma-derived endothelial cells (ODMECs). We isolated and cultured primary ODMECs from epithelial ovarian carcinoma tissue. ODMECs had higher expression of endoglin and VEGFR-2, and also exhibited enhanced spontaneous formation of vessel-like structures in vitro. Transfection of siRNA targeting endoglin in ODMECs cells resulted in the reduction of the proliferation and tube formation. These results indicate that a subset of ODMECs display abnormal angiogenic properties and this phenotype was blocked by decreasing endoglin levels, suggesting endoglin is essential for stimulating angiogenesis, and targeting it may be an attractive approach to anti-angiogenesis therapy for ovarian carcinoma.
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影响因子：
5
作者：
V. Schellerer;R. Croner;Kristina Weinländer;W. Hohenberger;M. Stürzl;E. Naschberger
通讯作者：
V. Schellerer;R. Croner;Kristina Weinländer;W. Hohenberger;M. Stürzl;E. Naschberger
DOI：
10.1891/9780826121646.0002
发表时间：
2018-09
期刊：
Cancer Rehabilitation
影响因子：
--
作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
通讯作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
影响因子：
9.8
作者：
Odegaard, Elin;Davidson, Ben;Staff, Anne Cathrine
通讯作者：
Staff, Anne Cathrine
影响因子：
3.6
作者：
N. Mclaughlin;B. Annabi;K. Kim;J. Bahary;R. Moumdjian;R. Béliveau
通讯作者：
N. Mclaughlin;B. Annabi;K. Kim;J. Bahary;R. Moumdjian;R. Béliveau
影响因子：
8.2
作者：
C. Wheeler-Jones;C. Clarkin;C. Farrar;Pam Dhadda;P. Chagastelles;N. Nardi;P. Jones
通讯作者：
C. Wheeler-Jones;C. Clarkin;C. Farrar;Pam Dhadda;P. Chagastelles;N. Nardi;P. Jones