Rational Design of Chemical Ligands for Selective Mitochondrial Targeting
Rational Design of Chemical Ligands for Selective Mitochondrial Targeting
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DOI：
10.1021/bc400291f
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发表时间：
2013-09-01
影响因子：
4.7
通讯作者：
Kral, Vladimir
中科院分区：
文献类型：
--
作者：
Rimpelova, Silvie;Briza, Tomas;Kral, Vladimir
The rational design of molecules with selective intracellular targeting is a great challenge for contemporary chemistry and life sciences. Here, we demonstrate a rational approach to development of compartment-specific fluorescent dyes from the gamma-aryl substituted pentamethine family. These novel dyes exhibit an extraordinary affinity and selectivity for cardiolipin in inner mitochondrial membrane and possess excellent photostability, fluorescent properties, and low phototoxicity. Selective imaging of live and fixed mitochondria was achieved in various cell lines using nanomolar concentrations of these dyes. Their high localization specificity and low toxicity enables study of morphological changes, structural complexity, and dynamics of mitochondria playing a pivotal role in many pathological diseases. These far-red emitting dyes could also serve in a variety of biomedical applications.
