Menadione (Vitamin K3) decreases melanin synthesis through ERK activation in Mel-Ab cells
Menadione (Vitamin K3) decreases melanin synthesis through ERK activation in Mel-Ab cells
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DOI：
10.1016/j.ejphar.2013.08.018
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发表时间：
2013-10-15
影响因子：
5
通讯作者：
Kim, Dong-Seok
中科院分区：
文献类型：
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作者：
Kim, Eun-Hyun;Kim, Myo-Kyoung;Kim, Dong-Seok
Menadione 是一种合成维生素 K3 衍生物。在这里，我们研究了甲萘醌对黑色素生成及其相关信号通路的影响。我们的结果表明，甲萘醌治疗后黑色素含量以剂量依赖性方式显着降低。然而，甲萘醌治疗并没有直接降低酪氨酸酶活性。众所周知，Wnt 信号传导在黑色素合成的控制中发挥着重要作用。因此，我们测试了甲萘醌治疗对 CSK3 β 和 β-连环蛋白信号传导的影响，但发现甲萘醌不影响这些信号传导途径中的任何一个。我们还研究了 ERK 磷酸化的变化，这与黑色素调节有关。这些结果表明甲萘醌处理导致ERK磷酸化。此外，甲萘醌治疗降低了 MITF 和酪氨酸酶蛋白水平。使用 P098059（一种特定的 ERK 通路抑制剂）进行治疗，可以恢复 menaclione 诱导的黑色素减少，并且还可以阻止 menaclione 引起的 WIT 和酪氨酸酶 clownregulaLion。这些结果表明，甲萘醌的低色素作用是通过 ERK 激活下调 MITF 和酪氨酸酶的线索。 (C) 2013 年爱思唯尔 BM。版权所有
Menadione is a synthetic vitamin K3 derivative. Here, we examined the effects of menadione on melanogenesis and its related signaling pathways. Our results showed that melanin content was significantly reduced after menadione treatment in a dose dependent manner. However, menadione treatment did not reduce tyrosinase activity directly. Wnt signaling is known to play a major role in the control of melanin synthesis. Thus, we tested the effects of menadione treatment on CSK3 beta and beta-catenin signaling, but found that menadione did not influence either of these signaling pathways. We also investigated changes in the phosphorylaLion of ERK, which is related to melanin regulaLion. These results indicated that menaclione treatment led to the phosphorylaLion of ERK. Additionally, menaclione treatment reduced both MITF and Lyrosinase protein levels. Treatment with P098059, a specific ERK pathway inhibitor, restored menaclionc-induced melanin reduction and also prevented WIT and Lyrosinase clownregulaLion by menaclione. These results suggest that the hypopigmenLary action of menadione is clue to MITF and Lyrosinase downregulaLion by ERK activation. (C) 2013 Elsevier BM. All rights reserved