Intersection of microwire electrodes with proximal CA1 stratum-pyramidale neurons at insertion for multiunit recordings predicted by a 3-D computer model
Intersection of microwire electrodes with proximal CA1 stratum-pyramidale neurons at insertion for multiunit recordings predicted by a 3-D computer model
复制标题
DOI：
10.1109/tbme.2004.834274
复制
发表时间：
2004-12-01
影响因子：
4.6
通讯作者：
Nadasdy, Z
中科院分区：
文献类型：
--
作者：
Claverol-Tinture, E;Nadasdy, Z
在神经元多单元细胞外记录的背景下，了解电极-组织相互作用以最大化可记录单元的数量并最小化由于机械组织改变而导致的实验伪影是广泛感兴趣的。为了实现这一目标，在海马CA 1区的微丝电极插入的计算机模型的开发，首先提供影响可记录（本地）神经元的电极-神经元交叉点的数量的估计，其次，以确定最佳的插入/电极参数，最大限度地减少交叉点的数量。该模型预测，在海马CA 1区，使用直径为50 μ m的电极，只有10%的可记录的神经元（那些在50 μ m的电极），将保持无碰撞。此外，该模型还预测，抑制性神经元不太容易被电极覆盖，导致无碰撞中间神经元的百分比比锥体细胞和中间神经元的相对密度所预期的高2至3倍。此外，该模型证实，与实验观察一致，相对于主神经元轴的电极倾斜增加了完整神经元的数量（与0度相比，45度时50 μ m电极的四倍，即，垂直于细胞体层的插入）。
It is of broad interest in the context of neuronal multiunit extracellular recordings to understand electrode-tissue interactions in order to maximize the number of recordable units and to, minimize experimental artifacts due to mechanical tissue alteration. Toward this goal, a computer model of microwire electrode insertion in hippocampus CA1 area was developed, firstly to provide estimates of the number of electrode-neuron intersections affecting recordable (local) neurons and, secondly, to determine optimal insertion/electrode parameters that minimize the number of intersections. The model predicts that in hippocampus CA1 area, using an electrode 50 mum in diameter, only 10% of the recordable neurons (those within 50 mum of the electrode), would remain collision free. Moreover, the model also predicts that inhibitory neurons are less prone to be intersected by the electrode, resulting in a 2 to threefold higher percentage of collision-free interneurons than expected from the relative densities of pyramidal cells and interneurons. Furthermore, the model confirms, in agreement with experimental observations, that electrode tilting with respect to the main neuronal axis increases the number of intact neurons (fourfold for a 50-mum electrode at 45degrees when compared to 0degrees, i.e., an insertion normal to the cell body layer).