Histological and histochemical changes in the gonads and epididymides of the male Soft‐shelled turtle, Trionyx sinensis
Histological and histochemical changes in the gonads and epididymides of the male Soft‐shelled turtle, Trionyx sinensis
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DOI：
10.1111/j.1469-7998.1977.tb04570.x
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发表时间：
2010-05
影响因子：
2
通讯作者：
B. Lofts;H. Tsui
中科院分区：
文献类型：
--
作者：
B. Lofts;H. Tsui
中华鳖精巢的季节性变化表明，生精小管和间质组织的功能活动在时间上存在明显的分化。在3-4月繁殖期，间质细胞较大，无脂，核圆形肿胀，A5-3P-羟基类固醇脱氢酶试验呈强阳性反应。正如这种高雄激素分泌活性的组织化学表现所预期的那样，附睾在这一时期也高度发达。相反，生精小管退化，生精不活跃，大量类脂。受精是通过从附睾管排出的精子来实现的。在繁殖期之后，间质组织立即萎缩，变得强烈的脂样化，对3P-HSD检测为阴性。这种间质退化伴随着附睾腺的退化和伴随的生精小管活动的复发。后者很快就会耗尽他们的脂质，并且对3P-HSD检测呈强阳性。精子发生恢复，到5月底，生精小管充满了所有生发阶段的代表。在初秋，精子发生停止，随着附睾在重新发育的间质细胞雄激素分泌恢复的影响下恢复，精子从管腔排出到附睾管，在那里它们保留到下一个繁殖季节。季节性组织化学事件与基本的垂体-性腺对睾丸功能的调节有关。
The seasonal testicular changes in the Soft-shelled turtle, T. sinensis, show a well-marked temporal differentiation between the functional activity of the seminiferous tubules and the interstitial tissue. In the March-April breeding period, the interstitial cells are large and lipid-free, with rounded turgid nuclei and a strongly positive reaction to tests for A5-3P-hydroxysteroid dehydrogenase. As might be expected with such histochemical manifestations of high androgen secretory activity, the epididymis is also highly developed during this period. In contrast, the seminiferous tubules are regressed, spermatogenetically inactive, and heavily lipoidal. Fertilization is achieved by spermatozoa discharged from the epididymidal canals. Immediately following the breeding period, the interstitial tissue atrophies and becomes strongly lipoidal and negative to tests for 3P-HSD. This interstitial regression is accompanied by the regression of the epididymides and a concomitant recrudescence of seminiferous tubule activity. The latter rapidly become depleted of their lipid and become strongly positive to tests for 3P-HSD. Spermatogenesis resumes, and the seminiferous tubules become filled with representatives of all the germinal stages by the end of May. In early autumn there is a cessation of spermatogenesis, and, as the epididymides recover under the influence of a recovering androgen secretion by the redeveloping interstitial cells, the spermatozoa become evacuated from the tubule-lumina to the epididymidal canals, where they remain until the succeeding breeding season. The seasonal histochemical events are discussed in relation to the basic pituitary-gonadal regulation of testicular function.