Curcumin enhances anti-tumor immune response in tongue squamous cell carcinoma
Curcumin enhances anti-tumor immune response in tongue squamous cell carcinoma
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DOI：
10.1016/j.archoralbio.2018.04.015
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发表时间：
2018-08-01
影响因子：
3
通讯作者：
Hu, Jian
中科院分区：
文献类型：
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作者：
Liao, Feng;Liu, Li;Hu, Jian
目的：研究姜黄素对舌鳞癌（TSCC）的抗肿瘤免疫应答作用。实验设计：采用细胞系（Cal 27、FaDu）和动物模型（4NQO小鼠模型）。MTT法检测细胞增殖。采用免疫印迹法、免疫组织化学法和免疫荧光法检测蛋白表达。流式细胞术检测Treg和MDSC的数量。结果：PD-L1和p-STAT3Y75的表达在Fadu和cal27细胞系中受到依赖抑制。体内实验结果表明，姜黄素能显著抑制4NQO小鼠模型的肿瘤生长。体内PD-L1和p-STAT3Y75的表达也同样下降。此外，姜黄素治疗后，通过增加CD8阳性T细胞，减少Tregs和MDSCs，抗肿瘤免疫应答显著提高。结论：姜黄素在体外和体内均可抑制PD-L1和p- stat3y75的表达。此外，姜黄素治疗后，免疫抑制肿瘤微环境发生改变。提示姜黄素可有效促进TSCC的抗肿瘤免疫反应。
Purpose: This study evaluated the role of anti-tumor immune response of curcumin on tongue squamous cell carcinama (TSCC).Experimental design: Cell lines (Cal 27, FaDu) and animal model (4NQO mice model) were uesd in this study. The MTT assay was used to detecte cell proliferation. The Western blotting, immunohistochemistry and immunofluorescence were used to examine the protein expression. The flow cytometry was performed to determine the number of Treg and MDSC.Results: The expression of PD-L1 and p-STAT3Y75 were does-dependently inhibited in Fadu and Cal 27 cell line. The results of in vivo demonstrated that curcumin significantly attenuated tumor growth in 4NQO mice model. The expression of PD-L1 and p-STAT3Y75 were similarly decreased in vivo. Moreover, the anti-tumor immune response was remarkably improved after curcumin treatment through increasing CD8 positive T cells and decreasing Tregs and MDSCs.Conclusions: Curcumin treatment resulted in inhibition of PD-L1 and p-STAT3Y7 5 expression both in vitro and in vivo. Moreover, the immunosuppressive tumor microenvironment was changed after curcumin treatment. These data suggested that curcumin could effectively promote anti-tumor immune response in TSCC.