Immune-relevant (including acute phase) genes identified in the livers of rainbow trout, Oncorhynchus mykiss, by means of suppression subtractive hybridization
Immune-relevant (including acute phase) genes identified in the livers of rainbow trout, Oncorhynchus mykiss, by means of suppression subtractive hybridization
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DOI：
10.1016/s0145-305x(00)00057-4
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发表时间：
2001-04-01
影响因子：
2.9
通讯作者：
Yano, T
中科院分区：
文献类型：
--
作者：
Bayne, CJ;Gerwick, L;Yano, T
为了开发用于分析急性期反应的工具，我们使用了来自鳟鱼肝脏的cDNA的抑制性消减杂交，所述鳟鱼肝脏处于未受挑战的状态，并且在响应于注射用弗氏不完全佐剂乳化的弧菌蛋白的过程中。所得cDNA文库含有25个或更多个免疫相关基因的300-600 bp长片段。其中15种以前未报道过鲑鱼，12种从未在任何鱼类中发现。已知的急性时相蛋白包括血清淀粉样蛋白A、转铁蛋白和前小脑蛋白样蛋白;鳟鱼C-多糖结合蛋白I可能也是一种急性时相蛋白。补体系统（n = 5）和凝血系统（n = 3）的组件，以及凝集素，各种结合蛋白，一个假定的抗菌肽，趋化因子，抗氧化酶，以及一些可能的细胞表面受体和代谢和溶酶体酶的文库中表示。一个克隆非常类似于一组Toll样受体，包括人LL-I受体。三个cDNA似乎代表完整的开放阅读框。(C)2001爱思唯尔科技有限公司版权所有。
To develop tools for analysis of the acute phase response, we used suppression subtractive hybridization of cDNAs from the livers of trout in an unchallenged state and in the course of a response to injection with a Vibrio bacterin emulsified in Freund's Incomplete Adjuvant. The resulting cDNA library contains 300-600 bp long fragments of 25 or more immune-relevant genes. Fifteen were previously unreported for salmonids, and 12 were not known from any fish species. Known acute phase proteins include serum amyloid A, transferrin and precerebellin-like protein; trout C-polysaccharide-binding protein I is probably also an acute phase protein. Components of both the complement system (n = 5) and the clotting system (n = 3), as well as lectins, various binding proteins, a putative antibacterial peptide, a chemotaxin, an anti-oxidant enzyme, as well as some likely cell-surface receptors and metabolic and lysosomal enzymes are represented in the library. One clone closely resembles a group of Toll-like receptors, including the human LL-I receptor. Three cDNAs appear to represent complete open reading frames. (C) 2001 Elsevier Science Ltd. All rights reserved.