Secreted Factors from Stem Cells of Human Exfoliated Deciduous Teeth Directly Activate Endothelial Cells to Promote All Processes of Angiogenesis.
Secreted Factors from Stem Cells of Human Exfoliated Deciduous Teeth Directly Activate Endothelial Cells to Promote All Processes of Angiogenesis.
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DOI：
10.3390/cells9112385
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发表时间：
2020-10-31
期刊：
影响因子：
6
通讯作者：
Kamiya H
中科院分区：
文献类型：
--
作者：
Kato M;Tsunekawa S;Nakamura N;Miura-Yura E;Yamada Y;Hayashi Y;Nakai-Shimoda H;Asano S;Hayami T;Motegi M;Asano-Hayami E;Sasajima S;Morishita Y;Himeno T;Kondo M;Kato Y;Izumoto-Akita T;Yamamoto A;Naruse K;Nakamura J;Kamiya H
糖尿病是动脉粥样硬化和缺血性血管疾病的主要危险因素。近年来，再生医学有望成为治疗缺血性疾病的一种新方法。我们以前的研究报道了干细胞移植以旁分泌方式促进糖尿病神经病变和缺血性血管疾病的治疗性血管生成，但确切机制尚不清楚。因此，我们研究了干细胞分泌的因子是否对内皮细胞具有直接的有益作用以促进血管生成。在无血清DMEM中培养人脱落乳牙（SHED）干细胞48 h后收集可溶性因子作为条件培养基（CM）。SHED-CM在MTT测定中显著增加人脐静脉内皮细胞（HUVECs）的细胞活力，并且在伤口愈合和Boyden室测定中加速HUVECs迁移。在小鼠的Matrigel栓测定中，在含有SHED-CM或SHED悬浮液的栓中，原代内皮细胞的迁移数量显著增加。在管形成测定中，SHED-CM诱导HUVEC的复杂管状结构。此外，SHED-CM显着增加从原代大鼠主动脉的新血管形成，表明SHED-CM刺激原代内皮细胞，以促进全面的血管生成过程。SHED-CM的血管生成作用等于或大于VEGF的有效浓度。总之，SHED-CM直接刺激血管内皮细胞以促进血管生成，并且具有未来临床应用的前景。
Diabetes is a major risk factor for atherosclerosis and ischemic vascular diseases. Recently, regenerative medicine is expected to be a novel therapy for ischemic diseases. Our previous studies have reported that transplantation of stem cells promoted therapeutic angiogenesis for diabetic neuropathy and ischemic vascular disease in a paracrine manner, but the precise mechanism is unclear. Therefore, we examined whether secreted factors from stem cells had direct beneficial effects on endothelial cells to promote angiogenesis. The soluble factors were collected as conditioned medium (CM) 48 h after culturing stem cells from human exfoliated deciduous teeth (SHED) in serum-free DMEM. SHED-CM significantly increased cell viability of human umbilical vein endothelial cells (HUVECs) in MTT assays and accelerated HUVECs migration in wound healing and Boyden chamber assays. In a Matrigel plug assay of mice, the migrated number of primary endothelial cells was markedly increased in the plug containing SHED-CM or SHED suspension. SHED-CM induced complex tubular structures of HUVECs in a tube formation assay. Furthermore, SHED-CM significantly increased neovascularization from the primary rat aorta, indicating that SHED-CM stimulated primary endothelial cells to promote comprehensive angiogenesis processes. The angiogenic effects of SHED-CM were the same or greater than the effective concentration of VEGF. In conclusion, SHED-CM directly stimulates vascular endothelial cells to promote angiogenesis and is promising for future clinical application.
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影响因子：
6
作者：
Gupta PK;Krishna M;Chullikana A;Desai S;Murugesan R;Dutta S;Sarkar U;Raju R;Dhar A;Parakh R;Jeyaseelan L;Viswanathan P;Vellotare PK;Seetharam RN;Thej C;Rengasamy M;Balasubramanian S;Majumdar AS
通讯作者：
Majumdar AS
对医疗统计信息的自由使用的易于使用的软件“ EZR”的调查。
影响因子：
4.8
作者：
Kanda Y
通讯作者：
Kanda Y
影响因子：
5.8
作者：
Eckard J;Dai J;Wu J;Jian J;Yang Q;Chen H;Costa M;Frenkel K;Huang X
通讯作者：
Huang X
DOI：
10.1146/annurev-pathol-121808-102144
发表时间：
2010
期刊：
Annual review of pathology
影响因子：
--
作者：
Coppé JP;Desprez PY;Krtolica A;Campisi J
通讯作者：
Campisi J
影响因子：
5
作者：
Chang, Ming-Chau;Tsao, Ching-Hua;Hung, Shih-Chieh
通讯作者：
Hung, Shih-Chieh