A neuroepithelial wave of BMP signalling drives anteroposterior specification of the tuberal hypothalamus.
A neuroepithelial wave of BMP signalling drives anteroposterior specification of the tuberal hypothalamus.
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DOI：
10.7554/elife.83133
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发表时间：
2023-02-10
期刊：
影响因子：
7.7
通讯作者：
Placzek M
中科院分区：
文献类型：
--
作者：
Chinnaiya K;Burbridge S;Jones A;Kim DW;Place E;Manning E;Groves I;Sun C;Towers M;Blackshaw S;Placzek M
结节性下丘脑控制着维持生命的稳态过程，但尽管其具有基本作用，但在胚胎发生过程中指定中枢神经系统这一独特区域的细胞和信号通路的特征却很少。在这里，我们将实验和生物信息方法结合在胚胎鸡中，表明结节性下丘脑是由下丘脑底板样细胞逐渐产生的。命运图谱研究表明，随着一波神经上皮源性 BMP 信号从前部到后部穿过下丘脑底板，前腹神经管中会形成一串结节祖细胞。随着后来指定的后结节祖细胞的产生，早期指定的前结节祖细胞逐渐远离这些 BMP 信号并分化为结节神经源细胞。体内和离体功能获得和丧失的实验表明，BMP 信号传导启动结节祖细胞特化，但必须消除这些信号才能发展为前神经源性祖细胞。 scRNA-Seq 分析显示，在前结节规范主要时期后指定的结节祖细胞开始上调表征放射状胶质细胞的基因。这项研究提供了结节性下丘脑发育的综合说明。
The tuberal hypothalamus controls life-supporting homeostatic processes, but despite its fundamental role, the cells and signalling pathways that specify this unique region of the central nervous system in embryogenesis are poorly characterised. Here, we combine experimental and bioinformatic approaches in the embryonic chick to show that the tuberal hypothalamus is progressively generated from hypothalamic floor plate-like cells. Fate-mapping studies show that a stream of tuberal progenitors develops in the anterior-ventral neural tube as a wave of neuroepithelial-derived BMP signalling sweeps from anterior to posterior through the hypothalamic floor plate. As later-specified posterior tuberal progenitors are generated, early specified anterior tuberal progenitors become progressively more distant from these BMP signals and differentiate into tuberal neurogenic cells. Gain- and loss-of-function experiments in vivo and ex vivo show that BMP signalling initiates tuberal progenitor specification, but must be eliminated for these to progress to anterior neurogenic progenitors. scRNA-Seq profiling shows that tuberal progenitors that are specified after the major period of anterior tuberal specification begin to upregulate genes that characterise radial glial cells. This study provides an integrated account of the development of the tuberal hypothalamus.
影响因子：
3.6
作者：
Ratié L;Ware M;Barloy-Hubler F;Romé H;Gicquel I;Dubourg C;David V;Dupé V
通讯作者：
Dupé V
影响因子：
3.6
作者：
Ware M;Hamdi-Rozé H;Le Friec J;David V;Dupé V
通讯作者：
Dupé V