MicroRNA-214 suppresses the proliferation of human hepatocellular carcinoma cells by targeting E2F3
MicroRNA-214 suppresses the proliferation of human hepatocellular carcinoma cells by targeting E2F3
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DOI：
10.3892/ol.2015.3745
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发表时间：
2015-12-01
期刊：
影响因子：
2.9
通讯作者：
Huang, Chen
中科院分区：
文献类型：
--
作者：
Yang, Yang;Chang, Su'e;Huang, Chen
MicroRNAs(MiRNAs)是参与肿瘤发生的重要基因调控因子。以往的研究表明miR-214是一种肿瘤抑制因子，能够抑制人肝细胞癌(HCC)细胞生长。然而，miR-214抑制肿瘤发展的机制仍不清楚。在本研究中，观察到miR-214通过直接靶向肝癌细胞中的E2F转录因子3(E2F3)来抑制肿瘤的增殖。采用集落形成、细胞周期和增殖实验研究miR-214对细胞增殖的抑制作用。此外，还利用免疫印迹和双荧光素酶报告分析来评估E2F3是否是miR-214的靶标。这些分析结果表明，E2F3是miR-214的一个新靶点。此外，miR-214的表达增强或E2F3的沉默抑制了肝癌SMMC-7721细胞的增殖。这些发现表明miR-214通过靶向E2F3抑制肝癌的生长，可能为治疗人肝癌提供一种新的途径。
MicroRNAs (miRNAs) are important gene regulators that participate in tumorigenesis. Previous studies have implicated that miR-214 is a tumor suppressor that is capable of inhibiting human hepatocellular carcinoma (HCC) cell growth. However, the mechanism by which miR-214 suppresses tumor development remains unknown. In the present study, miR-214 was observed to suppress tumor proliferation by directly targeting E2F transcription factor 3 (E2F3) in HCC cells. Colony formation, cell cycle and proliferation assays were employed to study the tumor suppressor role of miR-214 in cell proliferation. In addition, western blotting and dual-luciferase reporter assays were used to evaluate whether E2F3 was a target of miR-214. The results of these analyses revealed that E2F3 was a novel target of miR-214. Furthermore, enhanced expression of miR-214 or silencing of E2F3 inhibited the proliferation of HCC SMMC-7721 cells. These findings suggest that miR-214 suppresses HCC growth by targeting E2F3, and may provide a novel approach for the treatment of human HCC.