Hypermethylated Epidermal growth factor receptor (EGFR) promoter is associated with gastric cancer.
Hypermethylated Epidermal growth factor receptor (EGFR) promoter is associated with gastric cancer.
复制标题
高甲基化表皮生长因子受体 (EGFR) 启动子与胃癌相关。
DOI：
10.1038/srep10154
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发表时间：
2015-05-11
影响因子：
4.6
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Weng X;Zhang H;Ye J;Kan M;Liu F;Wang T;Deng J;Tan Y;He L;Liu Y
表皮生长因子受体（Epidermal growth factor receptor，EGFR）是受体酪氨酸激酶ErbB家族的一员，在胃癌组织中呈高表达。然而，EGFR表达的调控机制仍不清楚。采用Sequenom EpiTYPER法检测了30例胃癌患者正常组织和肿瘤组织中EGFR基因启动子的甲基化状态。同时采用真实的时间定量PCR（qPCR）检测30例乳腺癌组织中EGFR的表达水平。值得注意的是，在肿瘤组织中发现EGFR启动子甲基化水平高于相应的相邻非癌组织。在我们的研究中检测到的区域I DMR和区域II DMR中，肿瘤组织显著高甲基化（P分别为2.7743E-10和2.1703E-05）。I区CpG_2表达与远处转移有关（P = 0.0323）。此外，结果显示，区域I和区域II中的相对EGFR表达与EGFR甲基化变化之间存在显著相关性（分别为P = 0.0004和0.0001）。我们的研究结果有助于表明胃癌中EGFR启动子的高甲基化，它可能是胃癌状态和进展的潜在表观遗传生物标志物。
Epidermal growth factor receptor (EGFR) is a member of the receptor tyrosine kinases ErbB family and it is found to be overexpressed in gastric cancer. However, the mechanism of the regulation of the EGFR expression is still unknown. We used the Sequenom EpiTYPER assay to detect the methylation status of the EGFR promoter in normal and tumour tissues of 30 patients with gastric cancer. We also carried out quantitative real time PCR (qPCR) to detect the expression level of EGFR in our 30 patients. Notably, increased methylation level at EGFR promoter was found in tumour tissues than the corresponding adjacent noncancerous. In both Region I DMR and Region II DMR detected in our study, tumor tissues were significantly hypermethylated (P = 2.7743E−10 and 2.1703E−05, respectively). Region I_⊿CpG_2 was also found to be associated with the presence of distant metastasis (P = 0.0323). Furthermore, the results showed a strongly significant association between the relative EGFR expression and the EGFR methylation changes in both Region I and Region II (P = 0.0004 and 0.0001, respectively). Our findings help to indicate the hypermethylation at EGFR promoter in gastric cancer and it could be a potential epigenetic biomarker for gastric cancer status and progression.
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影响因子：
158.5
作者：
Lynch, TJ;Bell, DW;Haber, DA
通讯作者：
Haber, DA
影响因子：
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作者：
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影响因子：
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3.7
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