Rapid actin-based plasticity in dendritic spines
Rapid actin-based plasticity in dendritic spines
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DOI：
10.1016/s0896-6273(00)80467-5
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发表时间：
1998-05-01
期刊：
影响因子：
16.2
通讯作者：
Matus, A
中科院分区：
文献类型：
--
作者：
Fischer, M;Kaech, S;Matus, A
树突棘被认为是大脑中突触可塑性的主要部位。与这一假设相一致的是，棘含有高浓度的肌动蛋白，这表明它们可能是能动的。为了研究这种可能性，我们从表达用绿色荧光蛋白标记的肌动蛋白（GFP-肌动蛋白）的海马神经元进行视频记录。该试剂结合到含有肌动蛋白的结构中，并允许活神经元中肌动蛋白动力学的可视化。在成熟的神经元中，GFP荧光的记录揭示了树突棘形状的大肌动蛋白依赖性变化，类似于从以前的研究中使用固定的组织推断。可见的变化发生在几秒钟内，这表明突触的解剖可塑性可以非常迅速。除了为结构可塑性提供分子基础外，树突棘中存在运动肌动蛋白暗示突触后元件是这种现象的主要部位。
Dendritic spines have been proposed as primary sites of synaptic plasticity in the brain. Consistent with this hypothesis, spines contain high concentrations of actin, suggesting that they might be motile. To investigate this possibility, we made video recordings from hippocampal neurons expressing actin tagged with green fluorescent protein (GFP-actin). This reagent incorporates into actin-containing structures and allows the visualization of actin dynamics in living neurons. In mature neurons, recordings of GFP fluorescence revealed large actin-dependent changes in dendritic spine shape, similar to those inferred from previous studies using fixed tissues. Visible changes occured within seconds, suggesting that anatomical plasticity at synapses can be extremely rapid. As well as providing a molecular basis for structural plasticity, the presence of motile actin in dendritic spines implicates the postsynaptic element as a primary site of this phenomenon.