INTERLEUKIN-7 IS A T-CELL GROWTH-FACTOR
INTERLEUKIN-7 IS A T-CELL GROWTH-FACTOR
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DOI：
10.1073/pnas.86.15.5923
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发表时间：
1989-08-01
影响因子：
11.1
通讯作者：
SHEVACH, EM
中科院分区：
文献类型：
--
作者：
CHAZEN, GD;PEREIRA, GMB;SHEVACH, EM
白细胞介素7（IL-7）是一种25-kDa的细胞因子，其被纯化，并且基于其刺激前B细胞增殖的能力克隆了其相应的cDNA。研究表明，IL-7还可以作为与Con A的共刺激物，通过诱导白细胞介素2（IL-2）的产生来促进T淋巴细胞的增殖。我们在这里证明，IL-7与佛波醇12-肉豆蔻酸酯13-乙酸酯的组合可以直接驱动纯化的T细胞的增殖，并且这种反应不受环孢菌素A或IL-2和IL-4的抗体的抑制。用佛波醇肉豆蔻酸酯乙酸酯和IL-2、IL-4或IL-7刺激T细胞制备T细胞以响应三种淋巴因子中的任何一种。虽然在体外由抗-CD 3或同种异体细胞激活的T细胞在用IL-7攻击时未能增殖，但在体内对相同刺激物致敏的T细胞在用IL-7体外再刺激时表现出显著的增殖反应。因此，IL-7既可以以IL-2非依赖性方式作为T细胞的生长因子，又可以作为诱导淋巴因子应答的感受态因子。通过在体内而不是体外刺激T细胞受体复合物诱导IL-7应答的能力提高了IL-7可能在体内T细胞生长和分化中发挥作用的可能性。
Interleukin 7 (IL-7) is a 25-kDa cytokine which was purified and its corresponding cDNA was cloned based upon its ability to stimulate the proliferation of pre-B cells. It has been shown that IL-7 can also function as a costimulator with Con A for the proliferation of T lymphocytes by inducing the production of interleukin 2 (IL-2). We demonstrate here that IL-7 in combination with phorbol 12-myristate 13-acetate can directly drive the proliferation of purified T cells and that this response is not inhibited by cyclosporine A or by antibodies to IL-2 and IL-4. Stimulation of T cells with phorbol myristate acetate and IL-2, IL-4, or IL-7 prepared T cells to respond to any of the three lymphokines. Although T cells activated in vitro by anti-CD3 or allogeneic cells failed to proliferate when challenged with IL-7, T cells primed in vivo to the same stimuli demonstrated a significant proliferative response when restimulated in vitro with IL-7. IL-7 can, therefore, function both as a growth factor for T cells in an IL-2-independent manner and as a competence factor for the induction of lymphokine responsiveness. The ability to induce IL-7 responsiveness via stimulation of the T-cell receptor complex in vivo, but not in vitro, raises the possibility that IL-7 may play a role in T-cell growth and differentiation in vivo.