Molecular cloning and expression of rhesus macaque and sooty mangabey interleukin 16: biologic activity and effect on simian immunodeficiency virus infection and/or replication.
Molecular cloning and expression of rhesus macaque and sooty mangabey interleukin 16: biologic activity and effect on simian immunodeficiency virus infection and/or replication.
复制标题
恒河猴和白眉白介素 16 的分子克隆和表达：生物活性及其对猿猴免疫缺陷病毒感染和/或复制的影响。
DOI：
10.1089/aid.1998.14.1323
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Hillyer,CD
中科院分区：
文献类型：
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作者：
Lee,ME;Adams,JW;Villinger,F;Brar,SS;Meadows,M;Bucur,SZ;Lackey3rd,DA;Brice,GT;Cruikshank,WW;Ansari,AA;Hillyer,CD
白细胞介素 16 (IL-16) 已被证明可以通过抑制 HIV 和 SIV mRNA 转录来减少 HIV 和 SIV 的复制。为了进一步评估其作用，我们比较了从易患疾病的恒河猴和抗病的乌白眉猴中克隆的 IL-16。将重组恒河猴 (rr) IL-16 与重组乌白眉猴 (rm)、人类和其他非人类灵长类动物 IL-16 序列进行比较，并评估其诱导趋化性和抑制混合淋巴细胞反应 (MLR) 的能力。此外，还评估了rrIL-16和rmIL-16对SIVmac251的抑制作用，SIVmac251在T细胞和单核细胞/巨噬细胞（双向性）中有效复制，并克隆了SIVmac239，它在T细胞（T向性）中有效复制。 rrIL-16 和 rmIL-16 与人 IL-16 的序列比较显示 >97% 的氨基酸同一性。 rrIL-16 的生物表征揭示了在恒河猴和人类细胞系统中有效诱导趋化性（p < 0.05）并显着抑制 MLR（73 ± 0.6%，p < 0.05）。使用rrIL-16和rmIL-16，感染SIVmac251的PBMC的p27抗原产量减少达70%（分别为p<0.05和p<0.01）。在感染 SIVmac239 的类似培养物中，rrIL-16 和 rmIL-16 分别使 p27 水平降低 96% 和 100%。这些数据证明了 rrIL-16 和 rmIL-16 的生物和抗病毒功能。
Interleukin 16 (IL-16) has been shown to diminish HIV and SIV replication through inhibition of HIV and SIV mRNA transcription. To evaluate its role further, we compared IL-16 cloned from disease-susceptible rhesus macaques and disease-resistant sooty mangabeys. Recombinant rhesus macaque (rr) IL-16 was compared with recombinant sooty mangabey (rm), human, and other nonhuman primate IL-16 sequences and evaluated for its ability to induce chemotaxis and inhibit the mixed lymphocyte response (MLR). Also, rrIL-16 and rmIL-16 were evaluated for suppression of SIVmac251, which replicates efficiently in T cells and monocyte/macrophages (dual tropic), and cloned SIVmac239, which replicates efficiently in T cells (T tropic). Sequence comparison of rrIL-16 and rmIL-16 with human IL-16 showed >97% amino acid identity. Biocharacterization of rrIL-16 revealed potent induction of chemotaxis (p< 0.05) and marked inhibition of MLR (73 ± 0.6%,p< 0.05) in rhesus and human cell systems. Using rrIL-16 and rmIL-16, p27 antigen production from PBMCs infected with SIVmac251 was decreased up to 70% (p< 0.05 andp< 0.01, respectively). In similar cultures infected with SIVmac239, rrIL-16 and rmIL-16 reduced p27 levels by 96 and 100%, respectively. These data demonstrate the biologic and antiviral functionality of rrIL-16 and rmIL-16.