Preparation of collagen/calcium phosphate multilayer sheet using enzymatic mineralization
Preparation of collagen/calcium phosphate multilayer sheet using enzymatic mineralization
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DOI：
10.1016/j.biomaterials.2003.12.057
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发表时间：
2004-11-01
期刊：
影响因子：
14
通讯作者：
Tanabe, T
中科院分区：
文献类型：
--
作者：
Yamauchi, K;Goda, T;Tanabe, T
制备多层片材（2-10层），其由交替累积的胶原和磷酸钙层组成，每层厚度为6-8 μ m。无机层矿化的碱性磷酸酶催化水解的水溶性磷酸酯在钙离子的存在下。在胶原蛋白上形成的磷酸钙作为羟基磷灰石（主要）和无定形磷酸钙的混合物进行测定。多层片材不仅机械强度高，而且在干燥状态下是透明和柔性的。此外，胶原/磷酸钙多层片材在水中不溶胀以保持原始形态。作为支架，在顶部具有磷酸钙层的片材支持L929成纤维细胞的附着和生长。结合理化和生物学性质，讨论了酶矿化和胶原/磷酸钙复合片材。(C)2004爱思唯尔有限公司保留所有权利。
The multilayer sheets (2-10 layers), which consisted of alternately cumulated collagen and calcium phosphate layers with the thickness of 6-8 mum in each layer, were prepared. The inorganic layer was mineralized by means of an alkaline phosphatase-catalyzed hydrolysis of water-soluble phosphate esters in the presence of calcium ions. The calcium phosphate, which was formed on the collagen, was assayed as a mixture of hydroxyapatite (main) and amorphous calcium phosphate. The multilayer sheets were not only strong mechanically but also semitransparent and flexible in a dry state. Furthermore, the collagen/calcium phosphate multilayer sheets did not swell in water to keep the original morphology. As a scaffold, the sheets having the calcium phosphate layer on the top supported the attachment and growth of L929 fibroblast cells. The enzymatic mineralization and the collagen/calcium phosphate composite sheets were discussed in conjunction with physicochemical and biological properties. (C) 2004 Elsevier Ltd. All rights reserved.