A microfluidic device to confine a single cardiac myocyte in a sub-nanoliter volume on planar microelectrodes for extracellular potential recordings
A microfluidic device to confine a single cardiac myocyte in a sub-nanoliter volume on planar microelectrodes for extracellular potential recordings
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DOI：
10.1039/b315648f
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发表时间：
2004-01-01
期刊：
影响因子：
6.1
通讯作者：
Baudenbacher, FJ
中科院分区：
文献类型：
--
作者：
Werdich, AA;Lima, EA;Baudenbacher, FJ
描述了一种混合芯片，其将在硅橡胶（PDMS）中制造的微流体网络与平面微电极相结合。它被用来测量细胞外电位从单一的成年小鼠心肌细胞在一个有限的细胞外空间。所记录的细胞外电位的变化是由与自发启动的细胞内钙波相关的跨膜电流引起的。通过压力梯度将单细胞捕获在100 μ l微室内，并通过连续灌注维持数小时。此外，还证明了药物局部递送至细胞的一部分。在电沉积铂黑之后，电极的阻抗降低了10至20倍。用铂黑电极从单细胞记录的生物电势显示噪声降低三倍，导致最大信噪比为15：1。在不同的细胞中观察到的频率和形状的细胞外电位的特征变化，这是最有可能由于在肌浆网Ca2+负荷的差异。我们的设备架构允许集成电化学和光学传感器进行多参数记录。
A hybrid chip is described which combines a microfluidic network fabricated in a silicone elastomer ( PDMS) with planar microelectrodes. It was used to measure extracellular potentials from single adult murine cardiac myocytes in a restricted extracellular space. The recorded variations in the extracellular potentials were caused by transmembrane currents associated with spontaneously initiated intracellular calcium waves. Single cells were trapped inside the 100 pl microchamber by pressure gradients and maintained for several hours by continuous perfusion. In addition, the localized delivery of drugs to a portion of the cell was demonstrated. The impedance of the electrodes was reduced by a factor of 10 to 20 after the electrodeposition of platinum black. Biopotentials recorded from single cells with platinum black electrodes showed a three-fold decrease in the noise, resulting in a maximum signal-to-noise ratio of 15:1. Characteristic variations in the frequency and shape of the extracellular potentials were observed among different cells which are most likely due to differences in the sarcoplasmic reticulum Ca2+ load. Our device architecture permits the integration of electrochemical and optical sensors for multiparameter recordings.