CORRELATION OF GROWTH CAPACITY OF HUMAN-TUMOR CELLS IN HARD AGAROSE WITH THEIR INVIVO PROLIFERATIVE CAPACITY AT SPECIFIC METASTATIC SITES
CORRELATION OF GROWTH CAPACITY OF HUMAN-TUMOR CELLS IN HARD AGAROSE WITH THEIR INVIVO PROLIFERATIVE CAPACITY AT SPECIFIC METASTATIC SITES
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DOI：
10.1093/jnci/81.18.1406
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发表时间：
1989-09-20
影响因子：
10.3
通讯作者：
FIDLER, IJ
中科院分区：
文献类型：
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作者：
LI, LM;PRICE, JE;FIDLER, IJ
本研究的目的是确定在增加琼脂糖浓度的人肿瘤细胞的锚定非依赖性生长的程度是否与细胞在裸鼠中产生实验性转移的能力相关。将来自亲本系和体内选择用于转移的变体以及体外克隆系的人黑素瘤、乳腺癌和结肠癌细胞接种到含有0.3%、0.6%、0.9%或1.2%琼脂糖的培养基中。这些细胞也被注射到裸鼠体内：伊曲康用于治疗黑色素瘤，注射到乳腺脂肪垫用于治疗乳腺癌，注射到脾脏用于治疗结肠癌。密集琼脂糖中肿瘤细胞集落的产生（> 0.6%）与肺（黑色素瘤、乳腺癌）或肝（结肠癌）中实验性转移的产生相关。我们认为，肿瘤细胞的非贴壁依赖性生长程度可以预测其生物学行为和体内转移潜能。因此，这项技术可能有助于从异质性人类肿瘤中分离转移瘤。
The purpose of this study was to determine whether the degree of anchorage-independent growth of human tumor cells in increasing concentrations of agarose correlated with the capacity of the cells to produce experimental metastases in nude mice. Human melanoma, breast carcinoma, and colon carcinoma cells from parental lines and variants selected in vivo for metastasis and in vitro cloned lines were plated into medium containing 0.3%, 0.6%, 0.9%, or 1.2% of agarose. These cells were also injected into nude mice: itravenously for melanoma, into the mammary fat pad for breast carcinoma, and into the spleen for colon carcinoma. Production of tumor cell colonies in dense agarose (> 0.6%) correlated with production of experimental metastases in the lung (melanoma, breast carcinoma) or liver (colon carcinoma). We conclude that the degree of anchorage-independent growth of tumor cells can predict their biological behavior and metastatic potential in vivo. Thus, this technique may be useful for the isolation of metastatic from heterogeneous human neoplasms.