Role of fungal trehalose and bacterial thiamine in the improved survival and growth of the ectomycorrhizal fungus Laccaria bicolor S238N and the helper bacterium Pseudomonas fluorescens BBc6R8
Role of fungal trehalose and bacterial thiamine in the improved survival and growth of the ectomycorrhizal fungus Laccaria bicolor S238N and the helper bacterium Pseudomonas fluorescens BBc6R8
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DOI：
10.1111/j.1758-2229.2010.00145.x
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发表时间：
2010-08-01
影响因子：
3.3
通讯作者：
Frey-Klett, P.
中科院分区：
文献类型：
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作者：
Deveau, A.;Brule, C.;Frey-Klett, P.
菌根辅助细菌荧光假单胞菌BBc6R8通过促进双色漆树菌S238N外生菌根的共生前生长和存活，促进了该菌的建立。关于外生菌根真菌对辅助细菌或参与相互作用的分子的影响，目前尚不清楚。在本研究中，我们监测了荧光假单胞菌BBc6R8在接种双色乳杆菌的土壤和对照土壤中的种群密度，发现外生菌核菌提高了辅助细菌的存活率。我们结合生长测量、化学诱导剂分析、高效液相分析和电子基因组分析，研究了参与这种相互促进生长效应的真菌和细菌代谢物的特性。我们发现海藻糖，一种在真菌菌丝中积累到高水平的二糖，化学吸引和促进辅助细菌的生长。同时，荧光假单胞菌BBc6R8产生的硫胺素浓度对真菌的体外生长有促进作用。总而言之，我们的数据表明，这两种微生物之间的相互作用对两个物种都有利，至少部分依赖于营养互惠。
P>The mycorrhiza helper bacterial strain Pseudomonas fluorescens BBc6R8 enhances the establishment of Laccaria bicolor S238N ectomycorrhizae by improving the pre-symbiotic growth and survival of the fungus. Nothing is known about the effect of the ectomycorrhizal fungus on the helper bacteria or the molecules that are involved in the interaction. In this study, we have monitored the population density of the helper strain P. fluorescens BBc6R8 in soils inoculated with L. bicolor and in control soils and found that the ectomycorhizal fungus improves the survival of the helper bacteria. We investigated the identity of the fungal and bacterial metabolites involved in this reciprocal growth-promoting effect using a combination of growth measurements, chemoattractant assays, HPLC and in silico genome analyses. We showed that trehalose, a disaccharide that accumulates to high levels in the fungal hyphae, chemoattracted and promoted the growth of the helper bacteria. Meanwhile, P. fluorescens BBc6R8 produced thiamine at concentrations that enhanced the fungal growth in vitro. Altogether our data indicate that the interaction between the two microorganisms is beneficial for both species and relies, at least in part, on trophic mutualism.