The apoptotic and autophagic effects of cast Au-Pt, and differently manufactured Co-Cr and cp-Ti on three-dimensional oral mucosal model.
The apoptotic and autophagic effects of cast Au-Pt, and differently manufactured Co-Cr and cp-Ti on three-dimensional oral mucosal model.
复制标题
DOI：
10.1016/j.msec.2020.111672
复制
发表时间：
2020-10
期刊：
影响因子：
--
通讯作者：
Yuan Liu;Q. Hu;Yu Pan;Yinghui Wang;Lei Jiang;Honglei Lin;Donghong Lin;Hui Cheng
中科院分区：
文献类型：
--
作者：
Yuan Liu;Q. Hu;Yu Pan;Yinghui Wang;Lei Jiang;Honglei Lin;Donghong Lin;Hui Cheng
数字化牙科金属材料的应用引起了人们对其生物相容性的关注。三维口腔黏膜模型（3D OMM）可以很好地评价生物相容性。在当前研究中，我们着手测量铸造金铂合金（Au-Pt）、不同制造的钴铬合金（Co-Cr）和商业纯钛（cp-Ti）浸提液中的金属离子释放水平。我们进一步测试了两种三维OMM支架材料，以确定更好的一个为后续工作。最后，我们基于3D OMM评估了铸造Au-Pt，以及不同制造的Co-Cr和cp-Ti对粘膜细胞的凋亡和自噬作用。我们发现，在构建三维OMM时，Matrigel表现出比牛脱细胞真皮基质更好的性能。因此，在随后的研究中，选择Matrigel来构建3D OMM。离子释放和生物学评估结果表明，首先，铸造Au-Pt和cp-Ti比铸造Co-Cr触发更少的早期凋亡细胞和离子释放，这意味着它们具有更好的化学稳定性和生物相容性;其次，数字化制造（包括CAD/CAM铣削和SLM）Co-Cr与铸造相比，在3D OMM上显示出显著更低的离子释放水平和更少的早期凋亡效应。虽然铸造纯钛释放的离子比CAD/CAM铣削的多得多，但制造方法对纯钛的凋亡作用没有影响。因此，我们认为数字化方法具有与传统铸造方法相同甚至更好的化学稳定性和生物相容性。第三，尽管在所有测试组中观察到增加的自噬水平，但到目前为止，没有证据表明测试金属彼此相比触发不同水平的自噬。此外，相关性分析表明，钴，钨，锰似乎是潜在的诱导凋亡和自噬作用的钴铬。
The application of digitally manufactured dental metals has aroused the attention on their biocompatibilities. Three-dimensional oral mucosal model (3D OMM) would provide excellent assessments to the biocompatibility. In the current study, we set to measure metal ion release levels in the extracts of cast gold-platinum alloy (Au-Pt), differently manufactured cobalt-chromium alloy (Co-Cr) and commercially pure titanium (cp-Ti). We further tested two scaffold materials of 3D OMM to determine the better one for the succedent work. Lastly, we evaluated the apoptotic and autophagic effects of cast Au-Pt, and differently manufactured Co-Cr and cp-Ti on mucosal cells based on 3D OMM. We found that, in the construction of 3D OMM, Matrigel showed better performance than bovine acellular dermal matrix. Thus, Matrigel was chosen to construct the 3D OMM in the succedent studies. The results of ion release and biological assessments showed that, firstly, cast Au-Pt and cp-Ti triggered less early apoptotic cells and ion release than cast Co-Cr, implying better chemical stability and biocompatibility of them; secondly, digitally manufactured (including CAD/CAM milling and SLM) Co-Cr showed significantly lower ion release levels and lesser early apoptotic effects on 3D OMM as compared to the cast one. Although cast cp-Ti released much more ions than CAD/CAM milling one, manufacturing methods had no impact on apoptotic effect of cp-Ti. Therefore, we believe that digital methods possess same or even better chemical stability and biocompatibility than conventional casting one. Thirdly, although increased autophagic levels are observed in all test groups, so far there is no evidence that the test metals trigger different levels of autophagy as compared to each other. In addition, correlation analysis indicates that Co, W, and Mn appear to be the potential inducements for the apoptotic and autophagic effects of Co-Cr.