Identification of the expressed MHC class II DQB gene of the Asiatic black bear, Ursus thibetanus, in Japan.
Identification of the expressed MHC class II DQB gene of the Asiatic black bear, Ursus thibetanus, in Japan.
复制标题
日本亚洲黑熊 Ursus thibetanus 表达的 MHC II 类 DQB 基因的鉴定。
作者：
Y. Yasukochi;Toshifumi Kurosaki;M. Yoneda;H. Koike
主要组织相容性复合体（MHC）基因的遗传多样性估计可能是评估传染性疾病免疫反应能力的重要工具。我们用RACE-PCR方法从日本的亚洲黑熊（Ursus thibetanus）中鉴定出一个接近全长表达的DQB序列。这是熊座ae中第一个这样的完整长度表达式。熊至少有一个功能性DQB位点。在系统进化树分析中，其DQB氨基酸序列与食肉目动物的DQB序列形成一个单系群，与加州海狮Zalophus californianus的DQB等位基因核苷酸序列相似性达90%。我们比较了藏羚羊与包括智人在内的其他几种哺乳动物的DQB氨基酸组成。已知对人类MHC基因具有重要功能的氨基酸残基在其他哺乳动物物种中也趋于保守，而β 1结构域的pbr在哺乳动物物种中具有异质性。从熊身上获得的DQB序列不仅没有假定的帧移或缺失，而且没有像在人类DQB分子中观察到的异常氨基酸突变。这表明熊的DQB序列是一个明显的功能性DQB等位基因。作为初步研究，我们从基因组DNA中对DQB等位基因的外显子2区域进行了测序，并成功扩增出DQB基因座的外显子2。我们的研究将为藏羚羊的保护遗传学以及哺乳动物MHC区域提供有用的信息。
Genetic diversity estimation of the major histocompatibility complex (MHC) gene may be an important tool in the assessment of immune response ability against infectious disease. We were able to identify a near full-length expressed DQB sequence by RACE-PCR method from the Asiatic black bear, Ursus thibetanus in Japan. This is the first such full length expression in the Ursidae. The bear had at least one functional DQB locus. In phylogenetic tree analysis its DQB amino acid sequence formed a monophyletic group with DQB sequences from members of the order Carnivora and had a 90% nucleotide sequence similarity with the DQB allele of the California sea lion, Zalophus californianus. We compared the DQB amino acid composition of U. thibetanus with those of several other mammalian species including Homo sapiens. Amino acid residues known to be functionally important for human MHC genes, tended to be also conserved among other mammalian species while PBRs in the beta1 domain were heterogeneous among mammalian species. The DQB sequence obtained from the bear had not only no putative frameshifts or deletions but also no abnormal amino acid mutations such as had been observed in human DQB molecules. This suggests that the bear DQB sequence was an apparently functional DQB allele. As a preliminary study, we sequenced the exon 2 region of DQB alleles from genomic DNA, and succeeded to amplify the exon 2 of DQB loci. Our study will provide useful information for conservation genetics of the U. thibetanus as well as more generally regarding the mammalian MHC region.
CD4-主要组织相容性复合物 II 类相互作用的破坏会阻碍体内 CD4(+) T 细胞的发育。
DOI：
10.1073/pnas.95.8.4493
发表时间：
1998
影响因子：
11.1
作者：
Riberdy,JM;Mostaghel,E;Doyle,C
通讯作者：
Doyle,C
DOI：
10.1073/pnas.85.23.8998
发表时间：
1988-12-01
影响因子：
11.1
作者：
FROHMAN, MA;DUSH, MK;MARTIN, GR
通讯作者：
MARTIN, GR
影响因子：
10.7
作者：
Austin L. Hughes
通讯作者：
Austin L. Hughes