Porcine reproductive and respiratory syndrome virus (PRRSV): Monoclonal antibodies detect common epitopes on two viral proteins of European and US isolates
Porcine reproductive and respiratory syndrome virus (PRRSV): Monoclonal antibodies detect common epitopes on two viral proteins of European and US isolates
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DOI：
10.1016/0378-1135(96)00047-8
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发表时间：
1996-08-01
影响因子：
3.3
通讯作者：
Weiland, E
中科院分区：
文献类型：
--
作者：
WieczorekKrohmer, M;Weiland, F;Weiland, E
用16株杂交瘤细胞系制备了抗猪繁殖与呼吸综合征（PRRSV）荷兰分离株的单克隆抗体（mAb）。用纯化的PRRSV免疫STU小鼠，诱导其对宿主细胞成分的免疫耐受，然后将其与SP2/0骨髓瘤细胞融合，获得杂交瘤细胞株。免疫印迹研究表明，单克隆抗体具有不同的蛋白特异性。一个mAb与病毒15 kD蛋白反应，11个针对40-50 kD蛋白，4个针对30-40 kD蛋白。抗15 kD推定核衣壳蛋白的mAb以及5种抗这些蛋白上的40-50 kD蛋白识别表位的mAb，这些蛋白在各种欧洲和美国PRRSV分离株中是保守的。
Sixteen hybridoma cell lines secreting monoclonal antibodies (mAbs) directed against two dutch isolates of the causative virus of Porcine Reproductive and Respiratory Syndrome (PRRSV) were produced. The hybridoma cells resulted from fusions of SP2/0 myeloma cells with splenocytes of STU mice immunized with purified PRRSV after induction of immunotolerance against host cell constituents.Screening of supernatant fluids was performed by an indirect immunofluorescence assay on PRRSV-infected porcine alveolar macrophages. Immunoblotting studies revealed that the mAbs had different protein specificities. One mAb reacted with a viral 15 kD protein, eleven were directed against a 40-50 kD protein, and four against a 30-40 kD protein. The mAb against the 15 kD putative nucleocapsid protein is well as five mAbs against the 40-50 kD protein recognized epitopes on these proteins which are conserved in various European and U.S. isolates of PRRSV.