MiR-15a contributes abnormal immune response in myasthenia gravis by targeting CXCL10
MiR-15a contributes abnormal immune response in myasthenia gravis by targeting CXCL10
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MiR-15a 通过靶向 CXCL10 导致重症肌无力异常免疫反应
DOI：
10.1016/j.clim.2015.12.009
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发表时间：
2016-03-01
影响因子：
8.6
通讯作者：
Yang, Huan
中科院分区：
文献类型：
--
作者：
Liu, Xiao-Fang;Wang, Run-Qi;Yang, Huan
miR-15 a可能与自身免疫相关。本研究旨在检测miR-15簇在重症肌无力（MG）患者外周血单个核细胞（PBMC）中的表达，探讨miR-15 a在MG发病中的作用。我们发现MG中所有miR-15簇的表达均降低，并且眼MG（oMG）中miR-15 a水平更低，而MG中CXCL 10的产生增加。结论CXCL 10是MG中miR-15 a的功能靶基因。增加miR-15 a的表达可降低CXCL 10的表达，减轻免疫应答中T细胞的异常激活，而减少miR-15 a的表达可激活免疫应答异常。此外，刺激后miR-15 a表达显著降低，泼尼松治疗可上调激素反应性MG患者的miR-15 a表达。总之，我们的数据表明，减少miR-15 a的表达促进促炎细胞因子的产生，并有助于免疫反应，至少部分通过调节CXCL 10的表达在MG。(C)版权所有© 2016 Elsevier Inc.
MiR-15a is likely to be associated with autoimmunity. Here, we aimed to examine the expression of miR-15 cluster in PBMCs from myasthenia gravis (MG) patients and investigate the potential roles of miR-15a in MG. We found that the expression of all miR-15 cluster was decreased in MG, furthermore, miR-15a levels in ocular MG (oMG) were much lower, while CXCL10 production was increased in MG. We display that CXCL10 was a functional target gene of miR-15a in MG. Increasing miR-15a expression could reduce CXCL10 expression and alleviate the abnormal T cells activation in immune response, while decreasing miR-15a expression could activate immune response abnormally. Moreover, miR-15a expression was significantly decreased after stimulation, and prednisone treatment could upregulate miR-15a expression in steroid-responsive MG patients. Take together, our data suggest that decreased miR-15a expression facilitates proinflammatory cytokines production and contributes to immune response at least in part via regulating CXCL10 expression in MG. (C) 2016 Elsevier Inc All rights reserved.