Rapid plasticity follows whisker pairing in barrel cortex of the awake rat.
Rapid plasticity follows whisker pairing in barrel cortex of the awake rat.
复制标题
清醒大鼠的桶状皮质中的胡须配对后，可塑性迅速增强。
DOI：
10.1007/s00221-006-0644-y
复制
发表时间：
2007
影响因子：
2
通讯作者：
Ebner,FordF
中科院分区：
文献类型：
--
作者：
Sellien,Heike;Ebner,FordF
在啮齿动物的躯体感觉皮质中，突触的可塑性在整个生命过程中都很容易被诱导。修剪成年大鼠脸部一侧除两根胡须外的所有胡须，也就是所谓的胡须配对，会导致活跃的(完整的)胡须对各自桶柱中的皮质细胞产生更强的驱动力，而不活跃的(修剪过的)胡须的功效则被下调。到目前为止，这种依赖活动的可塑性是通过修剪除两根胡须之外的所有胡须来诱导的，让大鼠从1天到1个月探索他们的环境，然后在麻醉下对皮质反应进行生理学分析。这类研究增强了我们对皮质可塑性的了解，但麻醉使修剪胡须后最初几个小时发生的变化的检查复杂化。在这里，我们分析了在修剪胡须后几个小时内警觉、活跃的动物发生的短期变化。在以下几种条件下，测量了桶状皮层诱发反应的大小：(A)在空气中搅拌(对照)，(B)大鼠主动搅拌物体，(C)被动胡须刺激的时期，以确定大脑皮质中胡须配对可塑性变化的开始。在空气中无接触搅拌和被动晶须刺激的主要区别在于，前者对非活动切割的晶须刺激的反应增加，而后者对刺激的晶须的反应增加。这一结果支持了清醒、警觉的动物在修剪胡须后4小时内桶状皮质中出现胡须配对可塑性的结论。
Synaptic plasticity can be induced easily throughout life in the rodent somatic sensory cortex. Trimming all but two whiskers on one side of an adult rat’s face, called ‘whisker pairing’, causes the active (intact) whiskers to develop a stronger drive on cortical cells in their respective barrel columns, while inactive (trimmed) whisker efficacy is down-regulated. To date, this type of activity-dependent plasticity has been induced by trimming all but two whiskers, letting the rats explore their environment from 1 day to 1 month, after which cortical responses were analyzed physiologically under anesthesia. Such studies have enhanced our understanding of cortical plasticity, but the anesthesia complicates the examination of changes that occur in the first few hours after whisker trimming. Here we assayed the short-term changes that occur in alert, active animals over a period of hours after whisker trimming. The magnitude of barrel cortex evoked responses was measured in response to stimulation of the cut and paired whiskers of rats under several conditions: (a) whisking in air (control), (b) active whisking of an object by the rat, and (c) epochs of passive whisker stimulation to identify the onset of whisker pairing plasticity changes in cortex. The main difference between whisking in air without contact and passive whisker stimulation is that the former condition induces an increased response to stimulation of inactive cut whiskers, while the latter condition increases the responses to the stimulated whiskers. The results support the conclusion that whisker pairing plasticity in barrel cortex occurs within 4 h after whisker trimming in an awake, alert animal.